+ Translate
+ Most Popular
The pigeon tick (Argas reflexus): its biology, ecology, and epidemiological aspects
Prevalence of hemoglobin abnormalities in Kebili (Tunisian South)
Lipogranuloma: a preventable complication of dacryocystorhinostomy
Value of basal plasma cortisol assays in the assessment of pituitary-adrenal insufficiency
Bees from the Belgian Congo. The acraensis group of Anthophora
Placing gingival retraction cord
Total serum IgE, allergy skin testing, and the radioallergosorbent test for the diagnosis of allergy in asthmatic children
Acariens plumicoles Analgesoidea parasites des oiseaux du Maroc
Injuries of terminal phalanges of the fingers in children
Biology of flowering and nectar production in pear (Pyrus communis)
Das Reliktvorkommen der Aspisviper (Vipera aspis L.) im Schwarzwald
Hydrological modelling of drained blanket peatland
Pathologic morphology and clinical significance of the anomalous origin of the left circumflex coronary artery from the right coronary artery. General review and autopsy analysis of 30 cases
Cyto genetic analyses of lymphocyte cultures after exposure to calcium cyclamate
Axelrodia riesei, a new characoid fish from Upper Rio Meta in Colombia With remarks concerning the genus Axelrodia and description of a similar, sympatric, Hyphessobrycon-species
Favorable evolution of a case of tuberculosis of pancreas under antibiotic action
RIFM fragrance ingredient safety assessment, Valencene, CAS Registry Number 4630-07-3
Parenteral microemulsions: an overview
Temperate pasture: management for grazing and conservation
Evaluation of a new coprocessed compound based on lactose and maize starch for tablet formulation
Thermal expansion and cracking of three confined water-saturated igneous rocks to 800C
Revision of the genera of the tribe Stigmoderini (Coleoptera: Buprestidae) a discussion of phylogenetic relationships
Anal tuberculosis. Report of a case
Gastric tuberculosis in the past and present
Adaptive responses of the cardiovascular system to prolonged spaceflight conditions: assessment with Holter monitoring

An alternative approach for gene transfer in trees using wild-type Agrobacterium strains

An alternative approach for gene transfer in trees using wild-type Agrobacterium strains

Plant Molecular Biology 17(3): 441-452

ISSN/ISBN: 0167-4412

PMID: 1653060

DOI: 10.1007/bf00040638

Micropropagated shoots of three forest tree species, poplar (Populus tremula X P. alba), wild cherry (Prunus avium L.) and walnut (Juglans nigra X J. regia), were inoculated each with six different wild-type Agrobacterium strains. Poplar and wild cherry developed tumors that grew hormone-independently, whereas on walnut, gall formation was weak. On poplar and wild cherry, tumors induced by nopaline strains developed spontaneously shoots that had a normal phenotype and did not carry oncogenic T-DNA. From these observations, we have established a co-inoculation method to transform plants, using poplar as an experimental model. The method is based on inoculation of stem internodes with an Agrobacterium suspension containing both an oncogenic strain that induces shoot differentiation and a disarmed strain that provides the suitable genes in a binary vector. We used the vector pBI121 carrying neo (kanamycin resistance) and uidA (beta-glucuronidase) genes to facilitate early selection and screening. Poplar plants derived from kanamycin-resistant shoots that did not carry oncogenic T-DNA, were shown to contain and to express neo and uidA genes. These results suggest that wild-type Agrobacterium strains that induce shoot formation directly from tumors can be used as a general tool for gene transfer, avoiding difficult regeneration procedures.

Please choose payment method:

(PDF emailed within 0-6 h: $19.90)

Accession: 002024107

Download citation: RISBibTeXText

Related references

Evidence of Biological Control of Agrobacterium tumefaciens Strains Sensitive and Resistant to Agrocin 84 by Different Agrobacterium radiobacter Strains on Stone Fruit Trees. Applied and Environmental Microbiology 55(3): 741-746, 1989

Genetic transformation of Chrysanthemum using wild type Agrobacterium strains; strain and cultivar specificity. Plant Cell Reports 9(9): 505-508, 1991

Transformation experiments in brassica with wild type strains and shooter mutants of agrobacterium tumefaciens. International Botanical Congress Abstracts 17: 184, 1987

Genetic transformation of potato tissue by wild strains of agrobacterium tumefaciens and agrobacterium rhizogenes. Vyestsi Akademii Navuk BSSR Syeryya Biyalahichnykh Navuk 6: 24-28, 1990

Horticultural characterization of Angelonia salicariifolia plants transformed with wild-type strains of Agrobacterium rhizogenes. Plant Cell Reports 21(10): 981-987, 2003

Strategies to produce T-DNA free CRISPRed fruit trees via Agrobacterium tumefaciens stable gene transfer. Scientific Reports 10(1): 20155, 2020

Influence of the agrobacterium radiobacter strain k84 nopaline plasmid pat84b on conjugal transfer of plasmids between agrobacterium strains. Phytopathology 79(8): 909-910, 1989

Changes in morphological phenotypes and essential oil components in lavandin (Lavandula x intermedia Emeric ex Loisel.) transformed with wild-type strains of Agrobacterium rhizogenes. Scientia Horticulturae 130(3): 647-652, 2011

Cardenolide accumulation in crown galls and cell lines obtained by transformation of Digitalis lanata with Agrobacterium tumefaciens wild-type strains. Planta medica 56(6): 498-499, 1990

Triazole and echinocandin MIC distributions with epidemiological cutoff values for differentiation of wild-type strains from non-wild-type strains of six uncommon species of Candida. Journal of Clinical Microbiology 49(11): 3800-3804, 2011

Different organization of the tRNA-gene-associated repetitive element, DRE, in NC4-derived strains and in other wild-type Dictyostelium discoideum strains. European Journal of Biochemistry 217(2): 627-631, 1993

Studies on gene transfer of shoot apical meristems by Agrobacterium-mediated genetic transformation in a progeny of Chinese wild Vitis pseudoreticulata. Vitis 52(4): 185-192, 2013

A gE-MGB-TaqMan real-time PCR to differentiate pseudorabies virus gE gene detected vaccine strains from wild-type strains. Zhongguo Yufang Shouyi Xuebao / Chinese Journal of Preventive Veterinary Medicine 29(7): 550-554, 568, 2007

Genetic transformation of Verticordia grandis (Myrtaceae) using wild-type Agrobacterium rhizogenes and binary Agrobacterium vectors. Plant Science Limerick 111(1): 51-62, 1995

Differentiation of RotaTeq ® vaccine strains from wild-type strains using NSP3 gene in reverse transcription polymerase chain reaction assay. Journal of Virological Methods 237: 72-78, 2016