EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

An expression-site-associated gene family of trypanosomes is expressed in vivo and shows homology to a variant surface glycoprotein gene



An expression-site-associated gene family of trypanosomes is expressed in vivo and shows homology to a variant surface glycoprotein gene



Molecular and Biochemical Parasitology 43.1: 1-16



Utilizing first-strand cDNA from different stages, a gene family was identified that is expressed in bloodstream form trypanosomes but not in cultured procyclic forms. This family of 50-100 genes, termed bloodstream-specific 1 (BS1), shares a chromosomal distribution pattern similar to the variant surface glycoprotein (VSG) genes and the expression-site-associated genes (ESAGs). The BS1 genes are expressed in several variants of Trypanosoma brucei brucei and in Trypanosoma brucei gambiense. Sequence analysis of five members of this gene family reveals the recently described ESAG 6 and ESAG 7 genes [1] as well as the ESAG X gene [2] to be members of this family. We have been unable to localize the BS1 gene product in the cell but show that chronically infected rabbit serum recognizes recombinant BS1 protein arguing for expression in vivo. Finally we note that the derived protein sequence for the BS1 genes suggests an evolutionary relationship with at least one variant surface glycoprotein gene, and hence these studies may provide clues to understanding the molecular origins of antigenic variation in trypanosomes.

(PDF emailed within 0-6 h: $19.90)

Accession: 002025431

Download citation: RISBibTeXText

PMID: 2290439

DOI: 10.1016/0166-6851(90)90125-6



Related references

Biological variation among african trypanosomes: I. Clonal expression of virulence is not linked to the variant surface glycoprotein or the variant surface glycoprotein gene telomeric expression site. Dna and Cell Biology 29(5): 215-227, 2010

Co-duplication of a variant surface glycoprotein gene and its promoter to an expression site in African trypanosomes. Journal of Biological Chemistry 272(39): 24637-24645, 1997

Analysis of a donor gene region for a variant surface glycoprotein and its expression site in African trypanosomes. Nucleic Acids Research 29(10): 2012-2019, 2001

Coordinate transcription of variant surface glycoprotein genes and an expression site associated gene family in Trypanosoma brucei. Cell 42(1): 173-182, 1985

Leaky transcription of variant surface glycoprotein gene expression sites in bloodstream african trypanosomes. Journal of Biological Chemistry 274(24): 16884-16893, 1999

In-vitro competition analysis of procyclin gene and variant surface glycoprotein gene expression site transcription in Trypanosoma brucei. Molecular and Biochemical Parasitology ch; 113(1): 55-65, 2001

High-resolution analysis of multi-copy variant surface glycoprotein gene expression sites in African trypanosomes. Bmc Genomics 17(1): 806-806, 2016

Stable variant-specific transcripts of the variant cell surface glycoprotein gene 1.8 expression site in Trypanosoma brucei. Molecular and Cellular Biology 8(2): 854-859, 1988

Variant surface glycoprotein gene expression site switches in Trypanosoma brucei. Journal of Biological Chemistry 261(13): 6056-6063, 1986

The promoter for a variant surface glycoprotein gene expression site in Trypanosoma brucei. Embo Journal 9(9): 2791-2801, 1990

Expression site activation in Trypanosoma brucei with three marked variant surface glycoprotein gene expression sites. Molecular and Biochemical Parasitology9: 225-235, 2002

Cloning and analysis of a variant surface glycoprotein gene expression site in trypanosoma brucei. Turner, M J And D Arnot (Ed ) Current Communications in Molecular Biology: Molecular Genetics Of Parasitic Protozoa; Meeting, Cold Spring Harbor, New York, Usa, October 1987 Xi+170p Cold Spring Harbor Laboratory: Cold Spring Harbor, New York, Usa Illus Paper 72-76, 1988

High homology between variant surface glycoprotein gene expression sites of Trypanosoma brucei and Trypanosoma gambiense. Molecular and Biochemical Parasitology 48.2: 199-210, 1991

Expression of a retroposon-like sequence upstream of the putative Trypanosoma brucei variant surface glycoprotein gene expression site promoter. Molecular and Cellular Biology 13(11): 7036-7044, 1993

Subnuclear localization of the active variant surface glycoprotein gene expression site in Trypanosoma brucei. Proceedings of the National Academy of Sciences of the United States of America, 95(21): 12328-12333, 1998