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Callus and cell culture in sugarbeet


, : Callus and cell culture in sugarbeet. Acta Horticulturae ( 280): 271-276

Inbred lines of 4 nuclear genotypes (1, 2, 12 and 22) in combination with either male-sterile (CMS) or normal (O-type; OT) cytoplasm were used. Cotyledons and young leaflets were cultured on medium supplemented with IAA and kinetin for callus induction and with (1) IAA or NAA and (2) benzyladenine (BA) or kinetin for shoot regeneration. Suspension cultures were initiated by inoculating 2 g callus tissue into 50 ml liquid medium.

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Related references

Nakashima, H.; Tabayashi, N.; Abe, J.; Shimamoto, Y., 1988: Tissue culture of sugarbeet. Effect of genotype on callus induction from seeds, callus proliferation and regeneration from callus. Seeds of 21 genotypes were cultured on MS medium containing 2 micro M IAA and 5 micro M benzyladenine. Callus induction was noted in all genotypes with rates varying from 6 to 71%, while the level of proliferation differed within and between genot...

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