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Callus formation and plant regeneration from protoplasts of rapid-cycling Brassica campestris L


, : Callus formation and plant regeneration from protoplasts of rapid-cycling Brassica campestris L. Cruciferae Newsletter ( 14-15): 106-107

One callus, produced by hypocotyl protoplast culture from a self-compatible rapid-cycling line of B. campestris (CrGC66), produced a very pale green shoot bud 9 weeks after protoplast isolation.


Accession: 002042142

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Related references

Schenck H.R.; Hoffmann F., 1979: Callus and root regeneration from mesophyll protoplasts of basic brassica species brassica campestris brassica oleracea and brassica nigra. Mesophyll protoplasts isolated from leaves of B. capestris L. 'Steinacher Winterrubsen', B. oleracea L. var. gongylodes 'Blauer Speck' and B. nigra L. (Koch), line 2051, Gottingen, were induced to divide and to form callusses a...

Christey, M.C.; Lentini, Z.; Earle, E.D., 1987: Protoplast isolation and callus formation from a cell suspension of rapid-cycling Brassica campestris L.. In experiments aimed at protoplast fusion and in vitro selection, the use of white friable callus as a protoplast source gave division frequencies of 5-10%, with an overall plating efficiency of 0.02%. The callus responded well to protoplast cultu...

Schenck, H.; Hoffmann, F., 1979: Callus and root regeneration from mesophyll protoplasts of basic Brassica species: Brassia campestris, Brassica oleracea and Brassica nigra. Zeitschrift fur Pflanzenzuchtung 82(4): 354-360

Cardi, T.; Earle, E.D., 1995: Plant regeneration from mesophyll protoplasts of 'rapid cycling' Brassica oleracea with 'Chiang' cytoplasm. Leaves from in vitro plantlets yielded an average of 2.6 x 107 protoplasts/g of fresh tissue. Plating efficiency was 0.15%. The number of calluses with shoots ranged from 0 to 41.5% (9.3% average). Most regenerated plants were diploid (60%). The r...

Kartha, K.; Michayluk, M.; Kao, K.; Gamborg, O.; Constabel, F., 1974: Callus formation and plant regeneration from mesophyll protoplasts of rape plants (Brassica napus L. cv. zephyr). Protoplasts from mesophyll cells of rape plants (Brassica napus L. cv. Zephyr) were isolated by enzymatic removal of cell walls in an osmoticum consisting of sorbitol and mannitol. A two-step enzyme treatment involving 0.5% each of Onozuka P1500 c...

Kartha K.K.; Michayluk M.R.; Kao K.N.; Gamborg O.L.; Constabel F., 1974: Callus formation and plant regeneration from mesophyll protoplasts of rape plants brassica napus cultivar zephyr. Plant Science Letters 3(4): 265-271

Schenck, H.R.; Hoffman, F., 1979: Callus and root regeneration from mesophyll protoplasts of basic Brassica species: B. campestris, B. oleracea and B. nigra. Mesophyll protoplasts were isolated from the leaves of the turnip rape Steinacher Winterrubsen [Steinach Winter turnip rape], the kohl rabi Blauer Speck [Blue Bacon] and the Brassica nigra line 2051 grown under sterile conditions. Cell division an...

Schenck, H.; Robbelen, G., 1982: Somatic hybrids by fusion of protoplasts from Brassica oleracea and Brassica campestris Bird rape, plant regeneration. Zeitschrift fur Pflanzenzuchtung = Journal of plant breeding 89(4): 278-288

Bauer, R., 1988: Protoplast manipulation in the genus brassica i. plant regeneration from leaf and callus protoplasts of different brassica oleracea varieties. Protoplasts were isolated by enzymatic removal of cell walls from leaf and callus material of several cabbage, cauliflower and Brussels sprouts cultivars. To obtain high yields of vital protoplasts and an efficient regeneration of plants different...

Oosawa, K.; Takayanagi, K., 1984: Protoplast approach in vegetable breeding ii. culture media for plant regeneration from mesophyll protoplasts of hakuran interspecific hybrid brassica campestris x brassica oleracea. Mesophyll protoplasts of hakuran cv. Shouren were isolated by two-step treatment with 0.1% pectolyase Y-23 and 1.0% cellulase Onozuka RS in 0.5 M mannitol at pH 5.7. Driselase was not essential. Various combinations of culture media were tested to...