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Callus formation from protoplasts of grapevines (Vitis spec.)

, : Callus formation from protoplasts of grapevines (Vitis spec.). Physiologia Plantarum 82(1): A17

Protoplasts isolated from different organs of genotypes grown in vitro were studied for competence for cell division and regeneration. Protoplasts from petioles and leaves of Riesling, Kerner, Muller Thurgau, Orion and Vidal showed low rates of cell wall formation and in many cases first cell division failed. In contrast, protoplasts derived from stems and roots began dividing in the first week of culture.

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Related references

Mii, M.; Zou, Y.M.; Sugiyama, T.; Yanagihara, S.; Iizuka, M., 1991: High frequency callus formation from protoplasts of vitis labruscana bailey and vitis thunbergii sieb. et zucc. by embedding in gellan gum. Protoplasts were isoalted from cell suspension cultures of Vitis labruscana Bailey cultivar 'Niagara' and cultured in B5 medium supplemented with 0.2 mg l-1 2,4-dichlorophenoxyacetic acid (2,4-D), 0.02 mg l-1 6-benzylaminopurine (BAP), 2...

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