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Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3


, : Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3. FEMS Microbiology Letters 80(1): 45-49

A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans postribosomal supernatant (PRS) supported poly(U)-dependent elongation, suggesting that the C. albicans lysate contained a soluble translation factor functionally analogous to the S. cerevisiae translation factor EF-3. The presence of EF-3 in C. albicans was confirmed by Western blotting using an antibody raised against S. cerevisiae EF-3. This antibody was also used to screen a selection of Candida species, all of which possessed EF-3 with molecular mass in the range of 110-130 kDa.

Accession: 002042454

PMID: 1855649

DOI: 10.1111/j.1574-6968.1991.tb04634.x

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Related references

Colthurst, D.R.; Santos, M.; Grant, C.M.; Tuite, M.F., 1991: Candida albicans and three other Candida species contain an elongation factor structurally and functionally analogous to elongation factor 3. A cell-free poly(U)-dependent translation elongation system from Candida albicans is ATP-dependent due to the presence of an elongation factor 3 (EF3)-like activity. Saccharomyces cerevisiae ribosomes added to a C. albicans postribosomal supernata...

Colthurst D.R.; Santos M.; Grant C.M.; Tuite M.F., 1991: Candida albicans and three other candida spp contain an elongation factor structurally and functionally analogous to elongation factor 3. FEMS Microbiology Letters 80(1): 45-50

Slobin, L.I.; Moller, W., 1977: The heavy form of elongation factor 1 in artemia salina embryos is functionally analogous to a complex of bacterial factor elongation factor tu and elongation factor ts. The purified heavy form of elongation factor 1 (EF-1) from cysts of A. salina catalyzed the exchange of free GTP with a complex of EF-1L (EF-1.alpha.) and GDP. After heat treatment of EF-1H in the presence of GTP, the factor, while inactive by its...

Slobin, L.I.; Möller, W., 1978: Purification and properties of an elongation factor functionally analogous to bacterial elongation factor Ts from embryos of Artemia salina. A protein factor functionally analogous to bacterial EF-Ts was purified from developing embryos of the brine shrimp A. salina. The factor designated eEf-Ts, has properties similar to EF-1.beta. from pig liver. Specifically eEF-TS in these assay co...

Myers, K.K.; Fonzi, W.A.; Sypherd, P.S., 1992: Isolation and sequence analysis of the gene for translation elongation factor 3 from Candida albicans. Elongation factor 3 (EF-3) is a unique and essential component of the translational system in fungi. The gene, CEF-3, encoding elongation factor 3 has been isolated from the dimorphic fungus Candida albicans. A heterologous gene probe containing t...

Mendoza, A.; Serramia, M.J.; Capa, L.; Garcia Bustos, J.F., 1999: Translation elongation factor 2 is encoded by a single essential gene in Candida albicans. Translation elongation factor 2 (eEF2) is a large protein of more than 800 amino acids which establishes complex interactions with the ribosome in order to catalyze the conformational changes needed for translation elongation. Unlike other yeasts,...

Domínguez, J.M.; Martín, J.J., 1998: Identification of elongation factor 2 as the essential protein targeted by sordarins in Candida albicans. The target for sordarins in Candida albicans has been elucidated. Kinetic experiments of sordarin inhibition as well as displacement experiments showed that the formation of a sordarin-target complex follows a reversible mechanism. Binding of trit...

Mendoza, A.; Serramía, M.J.; Capa, L.; García-Bustos, J.F., 1999: Translation elongation factor 2 is encoded by a single essential gene in Candida albicans. Translation elongation factor 2 (eEF2) is a large protein of more than 800 amino acids which establishes complex interactions with the ribosome in order to catalyze the conformational changes needed for translation elongation. Unlike other yeasts,...

Swoboda, R.K.; Bertram, G.; Colthurst, D.R.; Tuite, M.F.; Gow, N.A.; Gooday, G.W.; Brown, A.J., 1994: Regulation of the gene encoding translation elongation factor 3 during growth and morphogenesis in Candida albicans. The level of the TEF3 mRNA, which encodes the fungal-specific translation elongation factor 3 (EF-3), was measured during the yeast-to-hyphal transition in Candida albicans. In contrast to a previous report, TEF3 mRNA levels were shown to change d...

Sundstrom, P.; Smith, D.; Sypherd, P.S., 1990: Sequence analysis and expression of the two genes for elongation factor 1a from the dimorphic yeast Candida albicans. Journal of Bacteriology 172: 36-45