+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs

, : Canine parvovirus empty capsids produced by expression in a baculovirus vector: use in analysis of viral properties and immunization of dogs. Journal of General Virology 73: 369-374

The VP-2 genes of canine parvovirus (CPV) and a recombinant consisting of CPV and feline panleukopenia virus (FPV) sequences were cloned into baculovirus expression vectors, fused to the baculovirus polyhedrin promoter. Recombinant baculoviruses were prepared and the properties of the parvovirus proteins expressed in insect cells examined. The proteins produced were the same size as the authentic CPV VP-2 protein, and were produced late after infection; the quantity of proteins recovered from the insect cell cultures was similar to those produced in CPV infections. Parvovirus particles formed had the haemagglutination (HA), sedimentation and buoyant density properties of authentic CPV capsids. Both the CPV capsids and the CPV-FPV recombinant capsids from the baculovirus system expressed the same epitopes as those seen in the viable parvoviruses when tested with a panel of anti-parvovirus monoclonal antibodies. Lysates of recombinant baculovirus-infected cells were inoculated into dogs, giving rise to serum neutralizing and HA-inhibiting antibodies, and the immunized dogs were protected from clinical disease upon challenge with a virulent isolate of the most recent antigenic type of CPV.

(PDF same-day service: $19.90)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002042608

PMID: 1371541

DOI: 10.1099/0022-1317-73-2-369

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

Cuzin, Béatrice., 2016: Alprostadil cream in the treatment of erectile dysfunction: clinical evidence and experience. Therapeutic Advances in Urology 8(4): 249-256

Hoehl, R., 2003: Neue Optionen der Therapie pulmonaler Pilzinfektionen beim nicht-neutropenischen Intensivpatienten. Pulmonary fungal infections in non-neutropenic ICU-patients are not frequent, however, their incidence is increasing with high morbidity and mortality, leading to prolonged stay in ICU wards and to excessive costs dependent on difficult delayed di...

Inaba, Kazuo, 2001: High level of protein ubiquitination in ascidian sperm. Sawada, H [Author], Yokosawa, H [Author], Lambert, C C [Author] The biology of ascidians 74-79

Lyon, Dj; Baltensperger, Dd; Blumenthal, Jm; Burgener, Pa; Harveson, Rm, 2004: Eliminating summer fallow reduces winter wheat yields, but not necessarily system profitability. Summer fallow is commonly used to stabilize winter wheat (Triticum aestivum L.) production in the Central Great Plains, but summer fallow results in soil degradation, limits farm productivity and profitability, and stores soil water inefficiently....

Clarke, C.Laura.; Keyes, S.Elizabeth.; Wilkinson, H.; Alexjuk, J.; Wilcockson, J.; Robinson, L.; Corner, L.; Cattan, M., 2016: Organisational space for partnership and sustainability: lessons from the implementation of the National Dementia Strategy for England. National policy initiatives are faced with challenges in their partnership development and sustainability. The National Dementia Strategy for England recommended Dementia Adviser (DA) and Peer Support Network (PSN) services and 40 demonstration si...

Filatov O. M., 1972: Most recent tectonics of the Atlas. English translation of Russian paper cited in this issue of the Bibliography 21 E73-00883.

Grayeli, Roozbeh; Hatami, Kianoosh, 2008: Implementation of the finite element method in the three-dimensional discontinuous deformation analysis 3D-DDA. A modified three-dimensional discontinuous deformation analysis (3D-DDA) method is derived using four-noded tetrahedral elements to improve the accuracy of current 3D-DDA algorithm in practical applications. The analysis program for the modified 3...

Gasche E., 1958: Dr. Otto Wilhelm Gutzwiller, 1889-1958. Biographic sketch of the Swiss-born petroleum geologist O. W. Gutzwiller, for many years a consulting geologists in Spain and, for briefer periods, in Venezuela, Peru, and elsewhere.

Vial, H.J.; Ancelin, M.L.; Thuet, M.J.; Philippot, J.R., 1988: Differential effects of chloroquine on the phospholipid metabolism of Plasmodium-infected erythrocytes. The effect of the antimalarial drug chloroquine (CQ) on the phospholipid metabolism in Plasmodium knowlesi-infected simian erythrocytes has been studied by incubating cells with different labeled precursors and various concentrations of CQ. The dr...

Kim, H.Yeol.; Dhong, H-Jong.; Chung, S.Kyu.; Chung, K.Whan.; Chung, Y.Jun.; Jang, K-Taek., 2007: Intranasal Helicobacter pylori colonization does not correlate with the severity of chronic rhinosinusitis. Otolaryngology--Head and Neck Surgery 136(3): 390-395