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Capsella bursa-pastoris cell suspension and protoplast cultures

, : Capsella bursa-pastoris cell suspension and protoplast cultures. Physiologia Plantarum 82(1): A10

This widespread cruciferous weed is frost tolerant and resistant to attack by the flea beetles Phyllotreta cruciferae and P. striolata (major pests of canola and mustard crops). With a view to using it as a gene source for introgression of useful traits into the Brassica crops through protoplast manipulation and somatic hybridization, fast-growing suspension cultures were established from leaf-derived callus. These proved to be good sources of viable protoplasts.

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