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Capture of chromosomal DNA by anti-histone antibodies for PCR


Nucleic Acids Research 19(12): 3459-3459
Capture of chromosomal DNA by anti-histone antibodies for PCR
DNA amplification by using the polymerase chain reaction (PCR) on whole blood, without DNA extraction, is unreliable. A method is described in which anti-histone antibodies adsorbed onto a polypropylene tube or polyvinyl microtitre well bind chromatin, and thus chromosomal DNA, from blood.


Accession: 002042827

PMID: 2062663

DOI: 10.1093/nar/19.12.3459



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