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Capture of chromosomal DNA by anti-histone antibodies for PCR


, : Capture of chromosomal DNA by anti-histone antibodies for PCR. Nucleic Acids Research 19(12): 3459-3459

DNA amplification by using the polymerase chain reaction (PCR) on whole blood, without DNA extraction, is unreliable. A method is described in which anti-histone antibodies adsorbed onto a polypropylene tube or polyvinyl microtitre well bind chromatin, and thus chromosomal DNA, from blood.

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Accession: 002042827

PMID: 2062663

DOI: 10.1093/nar/19.12.3459

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