+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Chemical detection of Z-DNA within the maize Adh1 promoter



Chemical detection of Z-DNA within the maize Adh1 promoter



Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 18(6): 1181-1184



Z-DNA is a left-handed helix which can form within tracts of alternating purines and pyrimidines. Tracts of potential Z-DNA identified by sequence inspection are often noted within regulatory portions of genes, but evidence that these tracts of sequence actually exist as Z-DNA is very limited, and not available for any plant gene. In this study, the chemical probes osmium tetroxide, diethylpyrocarbonate and hydroxylamine were used to show that a tract of alternating purines and pyrimidines in the Adh1 promoter (from -311 to -325) actually assumes a Z-DNA conformation under superhelical stress in vitro.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002048349

Download citation: RISBibTeXText

PMID: 1600153

DOI: 10.1007/bf00047722


Related references

Site-specific oligodeoxynucleotide binding to maize Adh1 gene promoter represses Adh1-GUS gene expression in vivo. Plant molecular biology: an international journal on molecular biology biochemistry and genetic engineering 19(5): 715-723, 1992

The maize Adh1 promoter: chromatin structure. Maize Genetics Cooperation Newsletter (60): 61-62, 1986

The maize Adh1 promoter : structures in supercoiled DNA. Maize Genetics Cooperation Newsletter (60): 62, 1986

Architecture of a plant promoter: S1 nuclease hypersensitive features of maize Adh1. Plant Molecular Biology 8(4): 299-307, 1987

The TATA box promoter region of maize Adh1 affects its organ-specific expression. Embo Journal 11(1): 157-166, 1992

Effect of the promoter and the first intron of maize Adh1 on foreign gene expression in rice. Maydica 35(4): 353-357, 1990

Osmium tetroxide footprinting of a scaffold attachment region in the maize Adh1 promoter. Plant Molecular Biology 22(6): 1145-1151, 1993

Promoter elements required for developmental expression of the maize Adh1 gene in transgenic rice. Plant Cell 6(6): 799-810, 1994

DNA insertion in the first intron of maize Adh1 affects message levels: cloning of progenitor and mutant Adh1 alleles. Proceedings of the National Academy of Sciences of the United States of America 81(13): 4125-4128, 1984

Isolation and characterization of proteins associated with the maize g-box binding complex recognizing a promoter element of the ADH1 gene. 1993

Developmental and environmental regulation of the maize alcohol dehydrogenase 1 (Adh1) gene: promoter-enhancer interactions. Plant gene systems and their biology Proceedings of a CIBA Geigy UCLA symposium held at Tamarron, Colorado, USA, 2-8 February 1987: 263-277, 1987