Continuous production of L-lactic acid from whey permeate by immobilized Lactobacillus casei subsp. casei

Krischke, W.; Schroder, M.; Trosch, W.

Applied Microbiology and Biotechnology 34(5): 573-578

1991


ISSN/ISBN: 0175-7598
DOI: 10.1007/bf00167901
Accession: 002058945

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Abstract
The production of L-lactic acid from whey permeate, a waste product of the dairy industry, by fermentation with the lactic acid bacterium Lactobacillus casei subsp. casei was investigated. A fermentation medium consisting of permeate and supplements, which enables exponential growth of the organisms, was developed. A fast method for determination of free and immobilized biomass in solid-rich media, based on measurement of cellular ATP, was evolved. Continuous fermentations in a stirred tank reactor (STR) and in a fluidized bed reactor (FBR) with immobilized biomass were compared. In the STR a volumetric productivity of 5.5 g/l per hour at 100% substrate conversion [dilution rate (D) = 0.22 h-1] was determined. In the FBR porous sintered glass beads were used for immobilization and a maximum biomass concentration of 105 g/kg support was measured. A productivity of 10 g/l per hour was obtained at D = 0.4 h-1 (substrate conversion 93%) and of 13.5 g/l per hour at D = 1.0 h-1 (substrate conversion 50%).

Continuous production of L-lactic acid from whey permeate by immobilized Lactobacillus casei subsp. casei