+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Coronavirus infection in the laboratory rat: immunization trials using attenuated virus replicated in L-2 cells

Coronavirus infection in the laboratory rat: immunization trials using attenuated virus replicated in L-2 cells

Canadian Journal of Veterinary Research 55(1): 60-66

Sixty-nine specific pathogen-free male Wistar rats approximately eight weeks of age were used to evaluate the efficacy of an attentuated strain of sialodacryoadenitis (SDA) virus in providing protection against infection on subsequent challenge with virulent SDA virus. Fifty-four animals were inoculated intranasally with approximately 10-1.5 median cell culture infectious doses of the 25th passage of SDA virus in L-2 cells. Randomly-selected vaccinated animals were killed in order to evaluate the safety and efficacy of attenuated virus by histopathological examination of the salivary glands, lacrimal glands, and lower respiratory tract, and titration of sera for antibody to SDA virus. At three months and six months post-vaccination (pv), animals were selected at random and challenged with virulent SDA virus. Seronegative, age-matched animals were also challenged, and served as controls. In animals examined at six to ten days pv, lesions were absent in submandibular and parotid salivary glands and lacrimal glands, but transient lesions were present in major airways of the lower respiratory tract. In a comparison of the incidence and extent of lesions, and antibody titers in challenged vaccinates and seronegative controls, lesions were minimal or absent in vaccinates compared to challenged naive rats, particularly in animals inoculated at three months pv. In addition, antibody titers in challenged vaccinates were much higher than were postinoculation titers in inoculated controls. In a comparison of lesions in salivary and lacrimal glands in vaccinated and control animals challenged at six months pv, there was a significant reduction in the number of animals without lesions in the vaccinated group (p = < 0.0001). Based on the incidence of lesions in target tissues and the marked antibody response in vaccinates on challenge with virulent SDA virus, it was concluded that prior inoculation with the attenuated strain of SDA virus provided a significant level of protection for up to six months postvaccination.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 002060695

Download citation: RISBibTeXText

PMID: 1653100

Related references

Long-term protection from SARS coronavirus infection conferred by a single immunization with an attenuated VSV-based vaccine. Virology 340(2): 174-182, 2005

Immunization of infants with living attenuated poliomyelitis virus; laboratory investigations of alimentary infection and antibody response in infants under six months of age with congenitally acquired antibodies. Journal of the American Medical Association 162(14): 1281-1288, 1956

Maternal immunization against coronavirus infection of calves: the mouse as a laboratory animal model. Berliner und Munchener Tierarztliche Wochenschrift 101(8): 278-282, 1988

Murine model for experimental studies on attenuated mutants of a Pasteurella multocida strain with pathogenicity for the calf. 2. Primary immunization against lethal infection, using fully attenuated streptomycin-dependent (Sm-d) mutant and booster immunization with partially attenuated streptomycin-dependent (Sm-id) revertants. Archiv für Experimentelle Veterinarmedizin 30(6): 845-851, 1976

Sequential immunization procedure against Group B arboviruses using living attenuated 17D yellow fever virus, living attenuated Langat E5 virus, and living attenuated dengue 2 virus (New Guinea C isolate). American Journal of Tropical Medicine and Hygiene 22(4): 509-523, 1973

Coronavirus infection in mink (Mustela vison). Serological evidence of infection with a coronavirus related to transmissible gastroenteritis virus and porcine epidemic diarrhea virus. Veterinary Microbiology 31(1): 1-10, 1992

Immunization of laboratory animals with ultraviolet-attenuated larvae against homologous challenge infection with Trichinella britovi. Southeast Asian Journal of Tropical Medicine and Public Health 29(3): 563-566, 1998

Immunization of guinea pigs against Argentinian hemorrhagic fever with Tacaribe virus replicated in human diploid cells. Medicina 41(4): 467-470, 1981

Protection against lethal Japanese encephalitis virus infection of mice by immunization with the highly attenuated MVA strain of vaccinia virus expressing JEV prM and E genes. Vaccine 17(3): 261-268, 1999

Induction of protective immunity against severe acute respiratory syndrome coronavirus (SARS-CoV) infection using highly attenuated recombinant vaccinia virus DIs. Virology 351(2): 368-380, 2006

Immunization against Argentina hemorrhagic fever using a strain attenuated Junin virus. II. Imunization of volunteers, clinical and laboratory analysis. Medicina 29(2): 81-87, 1969

Proteomics analysis of differentially expressed proteins in chicken trachea and kidney after infection with the highly virulent and attenuated coronavirus infectious bronchitis virus in vivo. Proteome Science 10: 24, 2012

Characteristics of Parker's rat coronavirus (PRC) replicated in L-2 cells. Archives of Virology 112(3-4): 195-202, 1990