EurekaMag.com logo
+ Site Statistics
References:
53,869,633
Abstracts:
29,686,251
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay



Detection of equine antibody to Babesia equi merozoite proteins by a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay



Journal of Clinical Microbiology 29(9): 2056-2058



A competitive inhibition enzyme-linked immunosorbent assay (CI ELISA) was developed to detect antibody to Babesia equi. One hundred fifty-four equine serum samples from 19 countries were tested for antibody to B. equi by the complement fixation test and by CI ELISA. The CI ELISA and complement fixation test results agreed in 94% (144) of the serum samples tested. The 10 discrepant serum samples were retested and analyzed for ability to immunoprecipitate in vitro translation products from B. equi merozoite mRNA. Five discrepant results were clearly resolved in favor of the CI ELISA, and the remaining five discrepancies were not definitively resolved.

(PDF emailed within 1 workday: $29.90)

Accession: 002067561

Download citation: RISBibTeXText

PMID: 1774334



Related references

Detection of equine antibody to babesia equi merozoite proteins by a monoclonal antibody based competitive inhibition elisa. Journal of Clinical Microbiology 29(9): 2056-2058, 1991

Monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detection of morbillivirus antibody in marine mammal sera. Journal of Clinical Microbiology 39(5): 1877-1881, 2001

Validation of a commercially available monoclonal antibody-based competitive-inhibition enzyme-linked immunosorbent assay for detection of serum antibodies to Neospora caninum in cattle. Journal of Clinical Microbiology 39(11): 3851-3857, 2001

Development of a monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay for detection of Bacillus piliformis isolate-specific antibodies in laboratory animals. Laboratory Animal Science 44(2): 153-158, 1994

A monoclonal antibody-based indirect competitive inhibition enzyme-linked immunosorbent assay for detecting tetrodotoxin in puffer fish. Wei Sheng Yan Jiu 26(2): 106-109, 1997

A monoclonal antibody-based competitive enzyme-linked immunosorbent assay for detecting antibody production against avian reovirus protein sigmaA. Journal of Virological Methods 136(1-2): 71-77, 2006

Development and evaluation of a monoclonal antibody based competitive enzyme-linked immunosorbent assay for the detection of rinderpest virus antibodies. Revue Scientifique et Technique 19(3): 754-763, 2000

Novel sensitive monoclonal antibody based competitive enzyme-linked immunosorbent assay for the detection of raw and processed bovine beta-casein. Plos One 12(7): E0182447-E0182447, 2017

Development of a new rabbit monoclonal antibody and its based competitive indirect enzyme-linked immunosorbent assay for rapid detection of sulfonamides. Journal of the Science of Food and Agriculture 93(3): 667-673, 2013

Development of a new monoclonal antibody based direct competitive enzyme-linked immunosorbent assay for detection of brevetoxins in food samples. Food Chemistry8: 2, 467-471, 2010

Serological diagnosis of bovine neosporosis by Neospora caninum monoclonal antibody-based competitive inhibition enzyme-linked immunosorbent assay. Journal of Clinical Microbiology 34(6): 1423-1428, 1996

Monoclonal antibody-mediated, immunodiagnostic competitive enzyme-linked immunosorbent assay for equine monocytic ehrlichiosis. Journal of Clinical Microbiology 27(1): 24-28, 1989

Development of a sensitive Indirect Competitive Enzyme-Linked Immunosorbent Assay (ic-ELISA) based on the monoclonal antibody for the detection of the imidaclothiz residue. Journal of Agricultural and Food Chemistry 59(5): 1594-1597, 2011

Detection of eastern equine encephalitis virus in infected mosquitoes using a monoclonal antibody-based antigen-capture enzyme-linked immunosorbent assay. American Journal of Tropical Medicine and Hygiene 65(3): 208-213, 2001

Development of monoclonal antibody-based ultrasensitive enzyme-linked immunosorbent assay and fluorescence-linked immunosorbent assay for 1-aminohydantoin detection in aquatic animals. Journal of Pharmaceutical and Biomedical Analysis 147: 417-424, 2017