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Differences between in vitro and in vivo obtained schistosomules

Differences between in vitro and in vivo obtained schistosomules

Revista do Instituto de Medicina Tropical de Sao Paulo 32(2): 84-85

The injection of cercariae of Schistosoma mansoni into the peritoneal cavity of naive mice induces cell adhesion to these larvae, and this adherence sharply decreases when the infecting larva changes to schistosomule. This procedure was used to detect differences between schistosomules obtained in vivo and in vitro. Reinoculation of schistosomules obtained in vivo into the peritoneal cavity of mice did not trigger cell adhesion. In contrast, adherent cells were found in 4 and 24-hour in-vitro schistosomules. Our data on schistosomules obtained in vitro indicate that more than 24 hours are needed for complete remotion of molecules involved in the phenomenon of cell adhesion.

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Accession: 002072249

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PMID: 2095629

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