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Differences in genomic DNA sequences between pathogenic and nonpathogenic isolates of Entamoeba histolytica identified by polymerase chain reaction



Differences in genomic DNA sequences between pathogenic and nonpathogenic isolates of Entamoeba histolytica identified by polymerase chain reaction



Journal of Clinical Microbiology 29(10): 2234-2239



A lambda gt11 cDNA library was constructed from the poly(A)+ RNA of trophozoites of E. histolytica HM-1:IMSS strain. The library was immunologically screened with MAb 4G6, which is specific for the 30 000 MW antigen of pathogenic isolates. A 0.7-kb clone was isolated, and its nucleotide sequence was determined.

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Accession: 002072290

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PMID: 1939576



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