+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Differential activation of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes by wounding and pathogen challenge



Differential activation of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes by wounding and pathogen challenge



Plant Cell 3(4): 397-405



Potato genes encoding 3-hydroxy-3-methylglutaryl coenzyme A reductase (HMGR) were expressed in response to pathogen, elicitor, and wounding. HMGR catalyzes the rate-limiting step in isoprenoid biosynthesis leading to accumulation of phytoalexins and steroid glycoalkaloids. Wounding caused increases in HMGR mRNA levels. A rapid and transient peak occurred 30 minutes after wounding, followed by a slower peak at 14 hours; both were correlated with increased enzyme activity. Induction of HMGR mRNA by the soft rot pathogen Erwinia carotovora subsp carotovora or arachidonic acid began 8 hours after challenge and continued through 22 hours. Potato HMGR is encoded by a gene family. An HMGR gene-specific probe was used to demonstrate that one isogene of the HMGR family is pathogen activated and is distinct from isogene(s) that are wound activated. This provides evidence that defense-related increases in HMGR activity are due to mRNA level increases and that HMGR isogenes are activated differentially by wounding or pathogen challenge.

(PDF emailed within 0-6 h: $19.90)

Accession: 002072508

Download citation: RISBibTeXText

PMID: 1840919

DOI: 10.1105/tpc.3.4.397


Related references

Isolation and differential regulation of two genes for 3 hydroxy 3 methylglutaryl coenzyme a reductase from potato. Plant Physiology 96(1 SUPPL): 6, 1991

Differential induction and suppression of potato 3-hydroxy-3-methylglutaryl coenzyme A reductase genes in response to Phytophthora infestans and to its elicitor arachidonic acid. Plant Cell 4(10): 1333-1344, 1992

Splice donor site mutations in the 3-hydroxy-3-methylglutaryl coenzyme A reductase gene cause a deficiency of the endoplasmic reticulum 3-hydroxy-3-methylglutaryl coenzyme A reductase protein in UT2 cells. Journal of Lipid Research 39(11): 2182-2191, 1998

Modulation of regulatory oxysterol formation and low density lipoprotein suppression of 3 hydroxy 3 methylglutaryl coenzyme a reductase activity by ketoconazole a role for cytochrome p 450 in the regulation of 3 hydroxy 3 methylglutaryl coenzyme a reductase in rat intestinal epithelial cells. Journal of Biological Chemistry 261(18): 8348-8356, 1986

Regulation of cytosolic acetoacetyl coenzyme A thiolase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl coenzyme A reductase, and mevalonate kinase by low density lipoprotein and by 25-hydroxycholesterol in Chinese hamster ovary cells. Journal of Biological Chemistry 255(16): 7787-7795, 1980

Differential regulation of the expression of 3-hydroxy-3-methylglutaryl coenzyme A reductase, synthase, and low density lipoprotein receptor genes. Journal of Lipid Research 33(8): 1157-1163, 1992

Identification of elements critical for phosphorylation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by adenosine monophosphate-activated protein kinase: protein engineering of the naturally nonphosphorylatable 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas mevalonii. Biochemistry 36(5): 1157-1162, 1997

Differential gene expression of phenylalanine ammonia lyase and 3-hydroxy-3-methylglutaryl coenzyme A reductase in potato leaves infected with US1 and US8 genotypes of Phytophthora infestans. Canadian Journal of Plant Pathology 25(4): 440, 2003

Increase in 3-hydroxy-3-methylglutaryl coenzyme A reductase mRNA level in tomato by fungal elicitors and mechanical wounding. Korean Journal of Plant Pathology 12(3): 285-290, 1996

Calcium ionophore treatment impairs the sterol mediated suppression of 3 hydroxy 3 methylglutaryl coenzyme a reductase 3 hydroxy 3 methylglutaryl coenzyme a synthase and farnesyl diphosphate synthetase. Journal of Biological Chemistry 267(4): 2831-2836, 1992

Coordinate regulation of 3-hydroxy-3-methylglutaryl-coenzyme A synthase, 3-hydroxy-3-methylglutaryl-coenzyme A reductase, and prenyltransferase synthesis but not degradation in HepG2 cells. Journal of Biological Chemistry 264(21): 12653-6, 1989

Transcriptional activation of the genes for low density lipoprotein ldl receptor and 3 hydroxy 3 methylglutaryl coenzyme a reductase hmg coa r in rat liver after chronic ethanol ingestion. Gastroenterology 98(5 PART 2): A600, 1990

Expression of a 3-hydroxy-3-methylglutaryl coenzyme A reductase gene from Camptotheca acuminata is differentially regulated by wounding and methyl jasmonate. Plant Physiology 103(1): 41-48, 1993

Regulation of 3-hydroxy-3-methylglutaryl-coenzyme A reductase by wounding and methyl jasmonate: Implications for the production of anti-cancer alkaloids. Plant Cell Tissue and Organ Culture 38(2-3): 351-356, 1994

Phosphorylation of 3-hydroxy-3-methylglutaryl coenzyme A reductase by microsomal 3-hydroxy-3-methylglutaryl coenzyme A reductase kinase. Archives of Biochemistry and Biophysics 230(1): 227-237, 1984