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Differential binding properties of protein A and protein G for dog immunoglobulins

Differential binding properties of protein A and protein G for dog immunoglobulins

Journal of Immunological Methods 145(1-2): 255-258

Binding of dog immunoglobulins G, A, M and E to protein A and protein G was investigated. Passive cutaneous anaphylaxis (PCA) testing was used for the measurement of dog IgE and ELISA were used for the measurements of dog IgG, IgA and IgM. Protein A from lyophilized cells of Staphylococcus aureus bound 97% of IgE, 98% of IgG, 81% of IgA and 97% of IgM. Protein A-Sepharose CL-4B bound 87% of IgE, 100% of IgG and IgA, and 98% of IgM.

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Accession: 002072524

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DOI: 10.1016/0022-1759(91)90335-d

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