EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Differential Detergent Stability of the Major Light-Harvesting Complex II in Thylakoids Isolated from Monocotyledonous and Dicotyledonous Plants



Differential Detergent Stability of the Major Light-Harvesting Complex II in Thylakoids Isolated from Monocotyledonous and Dicotyledonous Plants



Plant Physiology 99(3): 830-836



A survey of isolated thylakoids from 11 different higher plant species (Spinacia oleracea L., Pisum sativum L., Vicia faba L., Brassica napus L., Vigna sinensis L., Vinca minor L., Secale cereale L., Triticum aestivum L., Triticosecale Wittn., Hordeum vulgare L., Zea mays L.) indicated that the ratio of the oligomeric:monomeric form of the light-harvesting complex II was twofold higher for the dicots (3.16 +/- 0.35) than the monocots (1.64 +/- 0.25) examined under identical separation procedures. Under conditions specifically designed to stabilize the oligomeric form in vitro, we show that the oligomeric form of dicot light-harvesting complex II is twice as stable to solubilization in the presence of sodium dodecyl sulfate (SDS) than that observed for monocots. This decreased stability of monocot light-harvesting complex II is associated with a twofold increase in the trienoic fatty acid level of thylakoid phosphatidylglycerol but with no significant changes in the trienoic fatty acid levels in the major galactolipids. In addition, SDS polyacrylamide gel electrophoresis and western blot analyses with monoclonal antibodies indicated that monocots exhibited greater heterogeneity in the polypeptide complements associated with subfractions of light-harvesting complex II than the dicots examined. The data indicate that the oligomeric form of the light-harvesting complex II is not the result of a simple oligomerization of a common monomeric unit. We suggest that the difference in stability of the oligomeric form of light-harvesting complex II in isolated thylakoids of monocots and dicots is probably due to a differential accessibility to SDS. The differential SDS accessibility may be due to differences in thylakoid protein-protein and/or protein-lipid interactions.

(PDF same-day service: $19.90)

Accession: 002072543

Download citation: RISBibTeXText

PMID: 16669008

DOI: 10.1104/pp.99.3.830



Related references

Effects of high temperature exposure of spinach intact plants and isolated thylakoids on light-harvesting complex 2 protein phosphorylation. Biologia Plantarum 43(3): 433-435, 2000

Light-harvesting chlorophyll a/b-binding protein inserted into isolated thylakoids binds pigments and is assembled into trimeric light-harvesting complex. Plant Physiology 109(4): 1267-1276, 1995

High Yield Non-detergent Isolation of Photosystem I-Light-harvesting Chlorophyll II Membranes from Spinach Thylakoids: IMPLICATIONS FOR THE ORGANIZATION OF THE PS I ANTENNAE IN HIGHER PLANTS. Journal of Biological Chemistry 290(30): 18429-18437, 2015

The lateral migration of the light harvesting complex of photosystem 2 from chloroplast granal thylakoids to the stromal thylakoids as a function of light regimen in plant cultivation. Doklady Akademii Nauk Ukrainskoi SSR Seriya B Geologicheskie Khimicheskie i Biologicheskie Nauki (7): 57-60, 1986

Folding, assembly, and stability of the major light-harvesting complex of higher plants, LHCII, in the presence of native lipids. Biochemistry 39(46): 14305-14313, November 21, 2000

Stability of the Apoproteins of Light-Harvesting Complex I and II during Biogenesis of Thylakoids in the Chlorophyll b-less Barley Mutant Chlorina f2. Plant Physiology 107(3): 709-717, 1995

Macroorganization of chlorophyll a b light harvesting complex in thylakoids and aggregates information from circular differential scattering. Biochemistry 27(7): 2425-2430, 1988

The rate and extent of phosphorylation of the two light harvesting chlorophyll a b binding protein complex lhc ii polypeptides in isolated spinach thylakoids. Biochimica et Biophysica Acta 893(2): 333-341, 1987

Configuration and dynamics of xanthophylls in light-harvesting antennae of higher plants. Spectroscopic analysis of isolated light-harvesting complex of photosystem II and thylakoid membranes. Journal of Biological Chemistry 276(27): 24862-24870, 2001

The detergent and salt effect on the light-harvesting chlorophyll a/b complex from green plants. Biochimica et Biophysica Acta 636(2): 193-200, 1981

Inhibition of state transition and light-harvesting complex II phosphorylation-mediated changes in excitation energy distribution in the thylakoids of SANDOZ 9785-treated plants. Zeitschrift fuer Naturforschung Section C Biosciences 50(1-2): 77-85, 1995

Investigations of the role of the main light harvesting chlorophyll protein complex in thylakoid membranes reconstitution of depleted membranes from intermittent light grown plants with the isolated complex. Journal of Cell Biology 98(1): 163-172, 1984

Functional architecture of the major light-harvesting complex from higher plants. Journal of Molecular Biology 314(5): 1157-1166, 2001