+ Site Statistics
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Differential effects of omega-3 fish oils on protein kinase activities in vitro

Differential effects of omega-3 fish oils on protein kinase activities in vitro

American Journal of Physiology 261(1 Pt 1): E109-E114

We studied the in vitro effects of omega-3 fish oils and other fatty acids on the activity of crude protein kinase C from S49 lymphoma cells, on partially purified enzyme from rat cerebrum, on homogeneous protein kinase C from bovine brain, and, for comparison, on type I adenosine 3',5'-cyclic monophosphate (cAMP)-dependent protein kinase. In the absence of exogenous phospholipid, the fish oils cis-5,8,11,14,17-eicosapentaenoic acid (EPA) and acid (DCHA) enhance the catalytic cis-4,7,10,13,16,19-docosahexaenoic activity of protein kinase C and support the binding of [3H]phorbol 12,13-dibutyrate, both to approximately 50% of the level supported by phosphatidylserine. In the presence of phosphatidylserine, the omega-3 fatty acids reduce catalytic activity and [3H]phorbol 12,13-dibutyrate binding by about one-half. The effects of the omega-3 fatty acids on enzyme activity suggest that fish oils act as partial agonists competitively with phosphatidylserine. EPA, DCHA, and arachidonate (but not a variety of saturated fatty acids) inhibit the cAMP-dependent protein kinase. Thus dietary fish oils and cellular fatty acids mobilized by the action of phospholipase A2 may differentially modulate the activities of protein kinase C and cAMP-dependent protein kinase. These data suggest means by which unsaturated fatty acids mobilized within cells may act as second messengers.

(PDF same-day service: $19.90)

Accession: 002072596

Download citation: RISBibTeXText

PMID: 1858866

DOI: 10.1080/15298669191364686

Related references

Differential effects of omega 3 fish oils and other fatty acids on cyclic amp dependent protein kinase and protein kinase c. FASEB Journal 2(4): ABSTRACT 1737, 1988

Effects of dietary oils with extreme omega 3/omega 6 ratios--selective incorporation and differential catabolism. World Review of Nutrition and Dietetics 66: 195-204, 1991

Differential regulation of lipid and protein kinase activities of phosphoinositide 3-kinase gamma in vitro. Biological Chemistry 380(11): 1337-1340, 1999

Differential effects of suramin on protein kinase C isoenzymes: A novel tool for discriminating protein kinase C activities. FEBS Letters 421(2): 165-168, 1998

Direct Effects of Caffeine and Theophylline on p110d and Other Phosphoinositide 3-Kinases. Differential Effects on Lipid Kinase and Protein Kinase Activities. The Journal of Biological Chemistry 277(40): 124-30, 2002

Direct effects of caffeine and theophylline on p110 delta and other phosphoinositide 3-kinases. Differential effects on lipid kinase and protein kinase activities. Journal of Biological Chemistry 277(40): 37124-37130, 2002

Omega-3 fatty acids, inflammation and angiogenesis: basic mechanisms behind the cardioprotective effects of fish and fish oils. Cellular and Molecular Biology 56(1): 59-82, 2010

Omega-3 fatty acids, inflammation and angiogenesis: nutrigenomic effects as an explanation for anti-atherogenic and anti-inflammatory effects of fish and fish oils. Journal of Nutrigenetics and Nutrigenomics 1(1-2): 4-23, 2009

The cardiovascular effects of fish oils and omega-3 fatty acids. Heart Disease 1(3): 149-154, 2001

Modulation of omega-3/omega-6 polyunsaturated ratios with dietary fish oils in men with prostate cancer. Urology 58(2): 283-288, 2001

Cardiovascular effects of omega-3 polyunsaturated fatty acids (fish oils). Bailliere's Clinical Haematology 3(3): 625-649, 1990

Differential effects of polyamines on rat thyroid protein kinase activities. Journal of Cellular Biochemistry 28(4): 299-306, 1985

Insulin action in normal and protein kinase C-deficient rat hepatoma cells. Effects on protein phosphorylation, protein kinase activities, and ornithine decarboxylase activities and messenger ribonucleic acid levels. Molecular Endocrinology 1(1): 44-52, 1987

Differential in vitro effects of some pesticides on carbonic anhydrase activities from some freshwater and seawater fish erythrocytes. Fresenius Environmental Bulletin 13(1): 25-29, 2004