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Differential expression of Trypanosoma cruzi neuraminidase in intra- and extracellular trypomastigotes



Differential expression of Trypanosoma cruzi neuraminidase in intra- and extracellular trypomastigotes



Infection and Immunity 59(1): 464-466



The developmentally regulated expression of T. cruzi neuraminidase in culture cells was monitored by immunofluorescence with a MAb (TCN-2) against the enzyme. The results showed that TCN-2 reacted with all intracellular trypomastigote forms (NA+) but not with amastigotes. Immunoprecipitation of radiolabelled neuraminidase confirmed TCN-2 reactivity with trypomastigotes and the specificity of the antibody binding. During exiting from the host cells, all trypomastigotes were still NA+.

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Accession: 002072625

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PMID: 1987062



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