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Differential expression of a family of putative adenylate/guanylate cyclase genes in Trypanosoma brucei

Differential expression of a family of putative adenylate/guanylate cyclase genes in Trypanosoma brucei

Molecular and Biochemical Parasitology, 432: 279-288

The expression site for the variant surface glycoprotein (VSG) gene of Trypanosoma brucei contains several genes of unknown function (ESAGs, for expression site-associated genes). Among these, ESAG 4 shows homology to eukaryotic adenylate/guanylate cyclase genes, in the region encoding the presumptive enzyme catalytic domain. This gene belongs to a family of related sequences, and hybridizes to the genomic DNA of other trypanosomatids, such as Trypanosoma congolense, Trypanosoma vivax and Trypanosoma mega. While ESAG 4 is transcribed only in bloodstream forms by a RNA polymerase resistant to alpha-amanitin, at least three other members of this family are transcribed in both bloodstream and procyclic forms, by a RNA polymerase sensitive to the drug. These genes encode different putative transmembrane proteins showing high sequence conservation in the region corresponding to the adenylate/guanylate cyclase catalytic domain.

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Accession: 002072626

Download citation: RISBibTeXText

PMID: 1982555

DOI: 10.1016/0166-6851(90)90152-c

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