EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Differential Inhibition of Tonoplast H-ATPase Activities by Fluorescamine and Its Derivatives



Differential Inhibition of Tonoplast H-ATPase Activities by Fluorescamine and Its Derivatives



Plant Physiology 93(3): 1102-1109



Corn (Zea mays L.) root tonoplast vesicles were treated with the primary-amine specific reagent, fluorescamine (FL). Modification by FL caused a differential inhibition to the coupled activities of tonoplast H(+)-ATPase. Within the range of 0 to 5 micromoles of FL per milligram of protein, the proton pumping rate was significantly reduced but ATP hydrolysis was only slightly affected. Yet, the membrane H(+) leakage during the pumping stage increased only slightly. FL treatment resulted in (a) a decrease in amine containing phospholipids and (b) an insertion of multiple H-bonding moieties into the membrane. To test which of these two possible effects were responsible for inhibition, FL derivatives of benzylamine, butylamine, and phenylalanine were synthesized. It was found that the acyclic derivatives with high H-bonding potential at concentrations of 10 micromolar inhibited proton pumping by 50% without a significant effect on ATP hydrolysis. Cyclic derivatives were largely ineffectual. Proton leakage during pumping was not affected by these acyclic modifiers. Membrane fluidity, as measured by the polarization of diphenyl hexatriene, decreased upon treatment with either FL or its derivatives. The results suggest that the proton pumping is indirectly linked to ATP hydrolysis in the tonoplast vesicles, and the link between these processes is apparently weakened by the presence of acyclic fluorescamine derivatives in the membrane.

(PDF same-day service: $19.90)

Accession: 002072691

Download citation: RISBibTeXText

PMID: 16667564

DOI: 10.1104/pp.93.3.1102



Related references

Differential inhibition of tonoplast proton atpase activities by fluorescamine and its derivatives. Plant Physiology (Rockville) 93(3): 1102-1109, 1990

Differential inhibition of corn vanadate-sensitive H+-ATPase activities by fluorescamine and its derivatives. Plant Physiology & Biochemistry (Montrouge) 32(1): 93-104, 1994

Inhibition of vanadate sensitive proton atpase activities from plant roots by fluorescamine and its derivatives. Biophysical Journal 59(2 PART 2): 335A, 1991

Differential inhibition of tonoplast proton-ATPase activities by oryzalin. Biophysical Journal 64(2 PART 2): A353, 1993

Inhibition of tonoplast ATPase by 2',3'-dialdehyde derivatives of ATP. Plant physiology 98(1): 44-52, 1992

Differential inhibition of corn root tonoplast ATPase by dansyl chloride. Plant Physiology (Rockville) 102(1 SUPPL ): 104, 1993

Differential susceptibilities of tonoplast ATPase and PPase to irreversible inhibition by chaotropic anions. NATO advanced science institutes series: Series A: Life sciences: 4 179-186, 1987

Effects of salt stress on H+-ATPase and H+-ATPase activities of tonoplast-enriched vesicles isolated from sunflower roots. Physiologia Plantarum 97(2): 259-268, 1996

A chemical ionization mass spectrometric study of fluorescamine and fluorescamine amino acid derivatives. Analytical Letters 10(7-8): 575-580, 1977

Differential inactivation of beef heart mitochondrial f 1 atpase and itpase activities by the 3 o 4 benzoylbenzoyl derivatives of atp and itp. 1986

Indirectly coupled activities of tonoplast proton atpase. Biophysical Journal 57(2 PART 2): 351A, 1990

Differential effects on energy transduction processes by fluorescamine derivatives in rat liver mitochondria. Biochemistry 19(9): 1928-1933, 1980

Effects of calcium on the tonoplast ATPase and PPase activities from Acer pseudoplatanus. De Kouchkovsky, Y, INRA Colloquia; Plant sciences today: First general colloquium on plant sciences, Colloques de l'INRA; Plant sciences today: First general colloquium on plant sciences: 202, 1991

Irreversible inhibition of tonoplast atpase by chaotropic anions. Beilby, M J , N A Walker And J R Smith (Ed ) Membrane Transport in Plants And Fungi; 7th International Workshop on Plant Membrane Transport, Sydney, New South Wales, Australia, 1986 513p University Of Sydney: Sydney, New South Wales, Australia Illus Paper 194-198, 1990