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Differential mRNA transcription during the induction of freezing tolerance in spring and winter wheat



Differential mRNA transcription during the induction of freezing tolerance in spring and winter wheat



Plant and Cell Physiology 31(5): 609-619



Gene expression at the mRNA level was studied during cold hardening and dehardening of the freezing-tolerant cultivars Fredrick and Norstar and the freezing-sensitive cultivar Glenlea. Polyadenylated RNA isolated from crown and leaf tissues was translated in a wheat germ cell-free system and the acidic and basic in vitro products were resolved by isoelectric focusing and non-equilibrium pH gradient electrophoresis followed by SDS-PAGE.

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Accession: 002072699

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