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Differential migration during polyacrylamide gel electrophoresis suggests conformational differences among strains of potato spindle tuber viroid



Differential migration during polyacrylamide gel electrophoresis suggests conformational differences among strains of potato spindle tuber viroid



Canadian Journal of Plant Pathology 13(3): 202-211



Three strains of potato spindle tuber viroid (PSTVd) producing mild, intermediate-severe, and lethal symptoms, respectively, tomato plants were analyzed by polyacrylamide gel electrophoresis (PAGE). Under nondenaturing conditions, the band containing the circular and the linear RNA of the severe strain FS, 360 nucleotides (nt) long, migrated slightly faster than that of the RNAs of the severe-lethal strain FSL, 359 nt, followed by the still slightly slower moving band of the RNAs of the mild strain FM, 358 nt. Under denaturing conditions, where a slowly moving band of the circular RNA and a markedly faster moving band of the linear PSTVd RNA are found, the migration of the linear RNA of all three strains was practically identical, whereas the circular form of the mild strain FM and of the severe-lethal strain FSL migrated slightly faster than that of the severe strain FS. As a consequence, in "return"-PAGE (R-PAGE) the denaturing conditions of the "return" run did not alter the pattern of the differential migration of the circular molecules obtained in the nondenaturing run, so that the corresponding bands of the strains appeared in the same relative order as observed under nondenaturing conditions. The observed migration differences between the RNAs of the strains must be based on conformational differences that are effective under the various conditions of PAGE.

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Accession: 002072705

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DOI: 10.1080/07060669109500931



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