EurekaMag.com logo
+ Site Statistics
References:
53,623,987
Abstracts:
29,492,080
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on LinkedInFollow on LinkedIn

+ Translate

Differentially expressed isoforms of the mouse retinoic acid receptor beta are generated by usage of two promoters and alternative splicing



Differentially expressed isoforms of the mouse retinoic acid receptor beta are generated by usage of two promoters and alternative splicing



EMBO (European Molecular Biology Organization) Journal 10(1): 71-82



Using anchored PCR, three different cDNA isoforms of the mouse retinoic acid receptor .beta. [mRAR-.beta.1, mRAR-.beta.2 (formerly mRAR-.beta.0) and mRAR-.beta.3], generated from the same gene by differential promoter usage and alternative splicing, were isolated. These three isoforms encode RAR proteins with different N-terminal A regions and identical B-F regions. The sequence encoding the first 59 amino acids of the mRAR-.beta.3 A region is identical with the entire A region of mRAR-.beta.1. However, the sequence of mRAR-.beta.3 reigon A differs from that of mRAR-.beta. by an additional 27 C-terminal amino acids encoded in an 81 nucleotide-long putative exon which is spliced in between the exons encoding the A and B regions of mRAR-.beta.1. Both mRAR-.beta.1 and .beta.3 cDNAs differ entirely from mRAR-.beta.2 in their 5'-untranslated (5'-UTR) and A region coding sequences. This N-terminal variability, in a region which was shown to be important for cell-type specific differential target gene trans-activation by other nuclear receptors, suggests that the three mRAR-.beta. isoforms may be functionally distinct. The conservation of RAR-.beta. isoform sequences from mouse to human, as seen by cross-hybridization on Southern blot or DNA sequence analysis, as well as their differential patterns of expression in various mouse tissues, corroborates this view. Additionally, the mRNA analysis data suggest that mRAR-.beta.2, whose expression predominates in RA-treated embryonal carcinoma (EC) and embryonic stem (ES) cells, may be important during early stages of development. mRAR-.beta.1 and .beta.3, on the other hand, which are predominantly expressed in fetal and adult brain, may play some specific role in the development of the central nervous system.

(PDF 0-2 workdays service: $29.90)

Accession: 002072866

Download citation: RISBibTeXText



Related references

Differentially expressed isoforms of the mouse retinoic acid receptor beta generated by usage of two promoters and alternative splicing. Embo Journal 10(1): 71-81, 1991

Differentially expressed isoforms of the mouse retinoic acid receptor b are generated by usage of two promoters and alternative splicing. The EMBO Journal 10: 81, 1991

Differentially expressed messenger RNA isoforms of the human estrogen receptor-alpha gene are generated by alternative splicing and promoter usage. Molecular Endocrinology 12(12): 1939-1954, 1998

Two messenger RNA isoforms of the gonadotrophin-releasing hormone receptor, generated by alternative splicing and/or promoter usage, are differentially expressed in rainbow trout gonads during gametogenesis. Molecular Reproduction and Development 63(2): 151-160, 2002

Multiple isoforms of the mouse retinoic acid receptor a are generated by alternative splicing and differential induction by retinoic acid. The EMBO Journal 10: 69, 1991

Multiple isoforms of the mouse retinoic acid receptor alpha are generated by alternative splicing and differential induction by retinoic acid. Embo Journal 10(1): 59-69, 1991

RAR-beta 4, a retinoic acid receptor isoform is generated from RAR-beta 2 by alternative splicing and usage of a CUG initiator codon. Proceedings of the National Academy of Sciences of the United States of America 89(7): 2718-2722, 1992

Human and mouse DOCK10 splicing isoforms with alternative first coding exon usage are differentially expressed in T and B lymphocytes. Human Immunology 72(7): 531-537, 2011

Identification of Fca receptor (CD89) isoforms generated by alternative splicing that are differentially expressed between blood monocytes and alveolar macrophages. Journal of Immunology 156: 42-8, 1996

Identification of Fc alpha receptor (CD89) isoforms generated by alternative splicing that are differentially expressed between blood monocytes and alveolar macrophages. Journal of Immunology 156(11): 4442-4448, 1996

RAR-b4, a retinoic acid receptor isoform is generated from RAR-b2 by alternative splicing and usage of a CUG initiator codon. Proceedings of the National Academy of Sciences of the United States of America 89: 18-22, 1992

The mouse Rxrb gene encoding RXR beta: genomic organization and two mRNA isoforms generated by alternative splicing of transcripts initiated from CpG island promoters. Gene 142(2): 183-189, 1994

Identification of novel chicken estrogen receptor-alpha messenger ribonucleic acid isoforms generated by alternative splicing and promoter usage. Endocrinology 139(11): 4614-4625, 1998

Dominant negative effect of a novel form of the nuclear retinoic acid receptor-beta generated by alternative splicing. Proceedings of the American Association for Cancer Research Annual Meeting 34(0): 20, 1993