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Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro



Differentiation of pathogenic from nonpathogenic Entamoeba histolytica by restriction fragment analysis of a single gene amplified in vitro



Journal of Clinical Microbiology 29(2): 250-255



Homologous cDNA clones derived from a pathogenic isolate and a nonpathogenic isolate of E. histolytica have been previously reported and designated cEh-P1 and cEh-NP1, respectively. Sequence analysis of both clones revealed 10% nucleic acid substitutions, which were dispersed over the entire sequence. This genetic difference had been found to be conserved between all 4 pathogenic and all 5 nonpathogenic laboratory strains of E. histolytica tested.

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Accession: 002072952

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PMID: 1672533



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