+ Site Statistics
References:
52,654,530
Abstracts:
29,560,856
PMIDs:
28,072,755
+ Search Articles
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ PDF Full Text
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Translate
+ Recently Requested

Dissemination, replication, and trans-stadial persistence of Dugbe virus (Nairovirus, Bunyaviridae) in the tick vector Amblyomma variegatum



Dissemination, replication, and trans-stadial persistence of Dugbe virus (Nairovirus, Bunyaviridae) in the tick vector Amblyomma variegatum



American Journal of Tropical Medicine and Hygiene 45(1): 146-157



The dissemination and replication of Dugbe (DUG) virus and its tissue tropisms in the tick vector Amblyomma variegatum were examined by immunohistochemical analysis using specific antibody, in situ hybridization with a viral-complementary riboprobe, and infectivity assays of dissected tissues. Dugbe virus was localized in both unfed and feeding adults inoculated as nymphs or orally infected by capillary feeding, and in nymphs infected by capillary feeding. In non-feeding ticks, the main sites of DUG virus replication were the epidermis, hemocytes associated with loose connective tissue, and a small number of phagocytic digestive cells in the gut lumen. Virus infectivity in the hemolymph was associated entirely with hemocytes. Dugbe viral antigen or infectivity was not detected in the salivary glands until after the start of feeding. Viral titers in the salivary glands of feeding ticks were about ten-fold higher than in gut, ovary, or loose connective tissue. The level of infection decreased during molting and increased during feeding. Viral particles and pathologic effects were not detected in infected ticks. The primary site of trans-stadial persistence of DUG virus is the hemocytes. Tick hemocytes and other motile cells may be important in the transmission of persistent virus infection from one cell or organ to another by diapedesis.

(PDF emailed within 1 workday: $29.90)

Accession: 002074342

Download citation: RISBibTeXText

PMID: 1867347


Related references

Difference in vector competence of two species of sympatric ticks, Amblyomma variegatum and Rhipicephalus appendiculatus, for Dugbe virus (Nairovirus, Bunyaviridae). Virus Research 14(1): 73-84, 1989

Experimental studies on the replication and dissemination of qalyub virus bunyaviridae nairovirus in the putative tick vector ornithodoros erraticus. American Journal of Tropical Medicine & Hygiene 34(1): 180-187, 1985

Experimental studies on the replication and dissemination of Qalyub virus (Bunyaviridae: Nairovirus) in the putative tick vector, Ornithodoros (Pavlovskyella) erraticus. American Journal of Tropical Medicine and Hygiene 34(1): 180-187, 1985

Transovarian transmission in natura of Dugbe virus in the tick Amblyomma variegatum. Annales de l' Institut Pasteur, Virologie 138(2): 269-271, 1987

Transovarian passage of Dugbe virus in the tick Amblyomma variegatum (Fabricius). Bulletin de la Societe de Pathologie Exotique et de Ses Filiales 71(1): 19-22, 1978

Transovary passage of the Dugbe virus in the tick Amblyomma variegatum (Fabricius). Bulletin 71(1): 19-22, 1978

Dugbe virus replication in nymph and adult Amblyomma variegatum. Research in Virology 140(4): 333-336, 1989

Coding strategy of the S RNA segment of Dugbe virus (Nairovirus; Bunyaviridae). Virology 175(2): 518-524, 1990

Polypeptide synthesis of Dugbe virus, a member of the Nairovirus genus of the Bunyaviridae. Journal of General Virology 66: 141-148, 1985

Experimental infection of six species of ixodid ticks with Dugbe virus (family Bunyaviridae, genus Nairovirus). American Journal of Tropical Medicine and Hygiene 40(4): 410-417, 1989

Structure and morphogenesis of Dugbe virus (Bunyaviridae, Nairovirus) studied by immunogold electron microscopy of ultrathin cryosections. Virus Research 21(3): 199-212, 1991

Transovarial and trans-stadial transmission of Rickettsiae africae in Amblyomma variegatum ticks. Clinical Microbiology and Infection 15 Suppl 2: 317-318, 2010

Isolation of Dugbe virus from Amblyomma variegatum ticks in Cameroun. Transactions of the Royal Society of Tropical Medicine and Hygiene 68(5): 411-412, 1974

Transovarian transmission in nature of dugbe virus in amblyomma variegatum ticks. Annales de l'Institut Pasteur Virology 138(2): 269-272, 1987