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Early responses during plant-bacteria interactions in tobacco cell suspensions

Early responses during plant-bacteria interactions in tobacco cell suspensions

Phytopathology 81(12): 1504-1507

Bacteria that induce a hypersensitive response in tobacco leaves initiate a net uptake of extracellular H(+) and a net increase in extracellular K(+) in tobacco suspension cells. Other studies have shown that these interactions also result in a transient increase in lipid peroxidation and O2(-) production. The relationships between these early plant responses were studied simultaneously using suspension-cultured tobacco cells and compatible, incompatible, and saprophytic bacteria. The bathing medium was monitored during a 6-h period for pH, conductivity, [K(+)], and luminol-dependent chemiluminescence. In all bacterial treatments, an immediate increase in chemiluminescence and pH occurred within 30 min after addition of the bacteria. After about 2 h a second increase in pH, conductivity, [K(+)], and chemiluminescence occurred with incompatible bacteria. The first response appears nonspecific, while the second response appears to depend on the host/pathogen incompatibility.

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Accession: 002077443

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