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Effect of explant age and growth regulator concentration on adventitious shoot formation from cucumber cotyledonary tissue



Effect of explant age and growth regulator concentration on adventitious shoot formation from cucumber cotyledonary tissue



Report Cucurbit Genetics Cooperative ( 13): 14-17



Seeds of 2 varieties were used for the time course study and seeds of breeding line Gy14A for the secondary media study. For the former, cotyledons were excised at 2, 4, 6, 8 or 10 days of age and cultured on MS medium with supplements; for the later study, cotyledons of 5-day-old seedlings were used to derive explants (8-week-old cultured tissue) for culture on MS medium with 16 combinations of NAA and benzyladenine (BA).

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Accession: 002082162

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