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Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress

Plant Physiology 93(2): 471-478

Electrostatic Changes in Lycopersicon esculentum Root Plasma Membrane Resulting from Salt Stress

Salinity-induced alterations in tomato (Lycopersicon esculentum Mill. cv Heinz 1350) root plasma membrane properties were studied and characterized using a membrane vesicle system. Equivalent rates of MgATP-dependent H+-transport activity were measured by quinacrine fluorescence (pH gradient) in plasma membrane vesicles isolated from control or salt-stressed (75 millimolar salt) tomato roots. However, when bis-[3-phenyl-5-oxoisoxazol-4-yl] pentamethine was used to measure MgATP-dependent membrane potential (electrical potential difference) formation, salt-stressed vesicles displayed a 50% greater initial quench rate and a 30% greater steady state quench than control vesicles. This differential probe response suggested a difference in surface properties between control and salt-stressed membranes. Fluorescence titration of vesicles with the surface potential probe, 8-anilino-1-napthalenesulphonic acid (ANS) provided dissociation constants (Kd) of 120 and 76 micromolar for dye binding to control and salt-stressed vesicles, respectively. Membrane surface potentials of -26.0 and -13.7 millivolts were calculated for control and salt-stressed membrane vesicles from the measured Kd values and the calculated intrinsic affinity constant, Ki. The concentration of cations and anions at the surface of control and salt-stressed membranes was estimated using surface potential values and the Boltzmann equation. The observed difference in membrane surface electrostatic properties was consistent with the measured differences in K+-stimulated kinetics of ATPase activity between control and salt-stressed vesicles and by the differential ability of Cl- ions to stimulate H+-transport activity. Salinity-induced changes in plasma membrane electrostatic properties may influence ion transport across the plasma membrane.

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Accession: 002095329

PMID: 16667490

DOI: 10.1104/pp.93.2.471

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