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Factors affecting the survival of bisected sheep embryos in vivo

Factors affecting the survival of bisected sheep embryos in vivo

Theriogenology 36(3): 379-388

Two experiments were carried out to examine the effects of different factors on the survival of split sheep embryos. In Experiment 1, embryos collected on Day-6, Day-7 or Day-8 were bisected and transferred into recipient ewes in pairs. The proportions of Day-6, Day-7 and Day-8 demi-embryos developing to lambs were 26% (14/54), 30% (31/102) and 32% (24/74), respectively. Replacement of bisected late morula to expanded late blastocyst stage embryos into zonae did not affect their survival rate (P>0.05). The proportion of demi-embryos developing to lambs in recipients with two or more ovulations was higher (35%, 53/152) than in recipients with a single ovulation (21%, 16/78; P<0.05). In Experiment 2, Day-6 embryos were split with or without exposure to 0.25 M of sucrose and were transferred into recipients in pairs or singly. Exposure to 0.25 M of sucrose decreased the proportion of split embryos developing to lambs compared with that of the controls (31%, 22/70 vs 49%, 34/70; P<0.05). The effects of the number of demi-embryos transferred or the stage of development on the survival rate were not significant (P>0.05). The number of lambs born per original embryo was the highest when the embryos were split without exposure to sucrose and transferred into recipients singly (106%, 17/16).

Accession: 002106816

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PMID: 16727009

DOI: 10.1016/0093-691x(91)90466-q

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