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Haploid culture and in vitro mutagenesis (UV light, X-rays and gamma rays) of rapid cycling Brassica napus for improved resistance to disease



Haploid culture and in vitro mutagenesis (UV light, X-rays and gamma rays) of rapid cycling Brassica napus for improved resistance to disease



Plant mutation breeding for crop improvement: proceedings of an international symposium on the contribution of plant mutation breeding to crop improvement jointly organized by the International Atomic Energy Agency and the Food and Agriculture Organization of the United Nations and held in Vienna, 18-22 June 1990 Volume 2: 129-138



Microspores of rapid-cycling lines at the late uninucleate or early binucleate stage were released from anther walls and treated with gamma rays, X rays or ultraviolet (UV) light before incubation under standard microspore culture conditions. The frequency of cell division was an accurate early indication of the number of embryoids produced. The potential for plant regeneration was greatly reduced after treatment with gamma and X rays but not UV light.

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Accession: 002123174

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