High-resolution DNA amplification fingerprinting (DAF) : detection of amplification fragment length polymorphisms in soybean using very short arbitrary oligonucleotide primers

Caetano Anolles, G.; Bassam, B.J.; Gresshoff, P.M.

Soybean Genetics Newsletter 18: 279-283


Accession: 002125431

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DAF, which uses a single arbitrary primer as short as 5 nucleotides to amplify portions of a DNA template to give detailed and relatively complex DNA profiles, allows the easy detection of amplification fragment length polymorphisms (AFLPs) when amplification products are resolved by PAGE and stained with silver. The application of DAF to detect AFLPs in soyabean is described. DNA polymorphisms were detected between Glycine max and G. soja, and between soyabean cultivars. For example, as many as 6 AFLPs were detected between Bragg and Peking using the primer sequence CGCGGCCA.