EurekaMag
+ Most Popular
Cunninghamia lanceolata plantations in China
Mammalian lairs in paleo ecological studies and palynology
Studies on technological possibilities in utilization of anhydrous milk fat for production of recombined butter-like products
Should right-sided fibroelastomas be operated upon?
Large esophageal lipoma
Apoptosis in the mammalian thymus during normal histogenesis and under various in vitro and in vivo experimental conditions
Poissons characoides nouveaux ou non signales de l'Ilha do Bananal, Bresil
Desensitizing efficacy of Colgate Sensitive Maximum Strength and Fresh Mint Sensodyne dentifrices
Administration of fluid by subcutaneous infusion: revival of a forgotten method
Tundra mosquito control - an impossible dream?
Schizophrenia for primary care providers: how to contribute to the care of a vulnerable patient population
Geochemical pattern analysis; method of describing the Southeastern limestone regional aquifer system
Incidence of low birth weights in a hospital of Mexico City
Tabanidae
Graded management intensity of grassland systems for enhancing floristic diversity
Microbiology and biochemistry of cheese and fermented milk
The ember tetra: a new pygmy characid tetra from the Rio das Mortes, Brazil, Hyphessobrycon amandae sp. n. (Pisces, Characoidei)
Risk factors of contrast-induced nephropathy in patients after coronary artery intervention
Renovation of onsite domestic wastewater in a poorly drained soil
Observations of the propagation velocity and formation mechanism of burst fractures caused by gunshot
Systolic blood pressure in a population of infants in the first year of life: the Brompton study
Haematological studies in rats fed with metanil yellow
Studies on pasteurellosis. I. A new species of Pasteurella encountered in chronic fowl cholera
Dormancy breaking and germination of Acacia salicina Lindl. seeds
therapy of lupus nephritis. a two-year prospective study

Identification of tubulin isoforms in different tissues of Ascaris suum using anti-tubulin monoclonal antibodies


Identification of tubulin isoforms in different tissues of Ascaris suum using anti-tubulin monoclonal antibodies



International Journal for Parasitology 21(8): 913-918



ISSN/ISBN: 0020-7519

PMID: 1787032

DOI: 10.1016/0020-7519(91)90166-5

Three monoclonal antibodies specific to alpha- and beta-tubulin were used to examine the expression of tubulin isoforms in the intestine, reproductive tract and body wall muscle of A. suum. The tubulins were found to be different in their isoelectric points, number of isoforms and peptide maps with Western blot analysis of one-dimensional polyacrylamide gel confirming the presence of alpha-, beta 1- and beta 2-tubulin. Commercial cross-reactive anti-alpha and anti-beta MAbs 356 and 357 recognized tubulin from A. suum tissues as well as from pig brain, whereas anti-A. suum beta-tubulin specific MAb P3D6 recognized tubulin from the A. suum tissues only. Two-dimensional gel analysis showed different isoform patterns in different A. suum tissues with anti-A. suum beta-tubulin MAb P3D6 and cross-reactive beta-tubulin MAb 357 recognizing 2-4 beta-tubulin isoforms and anti-alpha-tubulin MAb 356 recognizing 1-6 alpha-tubulin isoforms. Different peptide maps of tubulin were observed in the three tissues, when subjected to limited proteolysis followed by SDS-PAGE. The data indicate that different tubulins are found in different tissues of adult A. suum.

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002129850

Download citation: RISBibTeXText

Related references

Tubulin isoforms recognized by an anti-tubulin monoclonal antibody K9. Cell Structure and Function 18(6): 642, 1993

Visualization of dimethyl sulphoxide-stabilized tubulin containing structures by fluorescence staining with monoclonal anti-tubulin antibodies. Histochemical Journal 16(4): 354-356, 1984

A monoclonal antibody to a-tubulin: purification of functionally active a-tubulin isoforms. Biochemistry (American Chemical Society) 38(17): 38-46, 1999

Characterization of the β-tubulin gene family in Ascaris lumbricoides and Ascaris suum and its implication for the molecular detection of benzimidazole resistance. Plos Neglected Tropical Diseases 15(9): E0009777, 2021

Differential immunocytochemical localisation of ?-tubulin and ?-tubulin in cerebellum using monoclonal antibodies. Cell Biology International Reports 6(11): 1047-1053, 1982

Differential immunocytochemical localisation of alpha-tubulin and beta-tubulin in cerebellum using monoclonal antibodies. Cell Biology International Reports 6(11): 1047-1053, 1982

Tubulin characterization during embryogenesis of Ascaris suum. Developmental Biology 76(1): 47-57, 1980

Tubulin characterization in embryos of Ascaris suum. Journal of Nematology 11(4): 298, 1979

A monoclonal antibody to alpha-tubulin: purification of functionally active alpha-tubulin isoforms. Biochemistry 38(17): 5438-5446, 1999

Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head. Histochemistry 95(5): 519-524, 1991

Anti-tubulin antibodies in a sensorimotor neuropathy patient alter tubulin polymerization. Acta Neuropathologica 95(3): 302-305, 1998

Chemical synthesis of five tubulin antigenic sequences. Production and characterization of their corresponding anti-tubulin monospecific antibodies. International Journal of Peptide and Protein Research 31(6): 555-566, 1988

Detection of private and recurrent idiotopes on natural anti-tubulin antibodies by monoclonal anti-idiotopic antibodies. Journal of Immunology 140(1): 148-154, 1988

Interaction of anthelmintic benzimidazoles with Ascaris suum embryonic tubulin. Biochimica et Biophysica Acta 630(2): 271-278, 1980

Interaction of anthelmintic benzimidazoles with Ascaris Suum embryonic tubulin. Biochimica et Biophysica Acta - General Subjects 630(2): 271-278, 1980