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Immunotrapping of Bacillus polymyxa in soil and in the rhizosphere of wheat

Immunotrapping of Bacillus polymyxa in soil and in the rhizosphere of wheat

Symbiosis Rehovot 9(1-3): 215-221

A serological method for immuno-isolation of Bacillus polymyxa from root-free soil and rhizosphere soil of wheat was developed. Microtiter plates were coated with rabbit polyclonal antibodies anti-B. polymyxa CF43 at 37 degrees C for 3 hours. Root-free soil or rhizosphere soil was added and incubated at 4 degrees C for 15 hours, and then microtiter plates were washed. The bound bacteria were desorbed with KCl 0.1M pH 5.5 and plated on sucrose agar medium.

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Accession: 002131184

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