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In vitro culture of immature soybean embryos. I. The abilities of somatic embryogenesis and regeneration from different aged immature embryos in Glycine max



In vitro culture of immature soybean embryos. I. The abilities of somatic embryogenesis and regeneration from different aged immature embryos in Glycine max



Journal of Agriculture and Forestry 38(2): 139-149



Immature embryos 3 to 30 days after pollination (DAP) were cultured on MSB or B5 media with added growth regulators. MSB medium with 0.1 mg NAA/litre gave better somatic embryogenesis than B5 medium with 0.1 mg IBA/litre. Callus, shoot and root formation were observed in 10-14 DAP embryos but not in younger embryos.

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Accession: 002133193

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