EurekaMag.com logo
+ Site Statistics
References:
53,214,146
Abstracts:
29,074,682
+ Search Articles
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Inhibition by diethylstilboestrol of proliferative potential of follicles of different sizes in immature rat ovaries



Inhibition by diethylstilboestrol of proliferative potential of follicles of different sizes in immature rat ovaries



Journal of Reproduction and Fertility 92(2): 323-332



Intact immature female rats were treated with 1, 2, 3 or 4 subcutaneous injections of 2 mg diethylstilboestrol (DES)/rat at intrevals of 24 h and then killed. Ovaries were collected, cleaned, enzymically digested and serially filtered through Telfon sieves to yield follicles of diameter < 200 .mu.m (small), 200-400 .mu.m (medium) and > 400 .mu.m (large). Follicular supernatant was collected and granulosa cells were extracted from these isolated follicles. There was a general increase in [3H]thymidine incorporation in all sizes of follicles after 1 or 2 DES injections, the increase in the medium and large follicles being significant after 2 doses. With 3 and 4 injections of DES, there was a sudden decrease in the rates of [3H]thymidine incorporation, particularly in the medium-sized follicles, which also had higher concentrations of follicular supernatant protein. Protein contents in small and large follicles did not change significantly. The follicular supernatant protein had a specific and dose-dependent inhibitory effect on [3H]thymidine incorporation when added to cultures of rapidly dividing granulosa cells. Addition of the same amounts of bovine serum albumin (BSA) to the cultures had no effect. Heat-denaturing did not abolish the inhibition by the protein. Removal of the protein from the cultures after the first 48 h resulted in a rebound increase in [3H]thymidine incorporation during the following 48 h, showing that the inhibitory effects were reversible. Though aromatase activity after 1 or 2 DES injections abruptly decreased after 3 and 4 injections, follicular supernatant protein had no effect on steroidogenesis in cultured granulosa cells. Taken together, these findings suggest that oestrogen can inhibit follicular development, depending on the duration of exposure. We propose that the inhibitory effects of DES on cell proliferation are mediated via the synthesis of a specific peptide factor which is produced in high amounts in the medium-sized follicles only, on prolonged exposure to the oestrogen. This factor may be autocrine or paracrine, serving as an in-built autoregulatory control mechanism for follicle development, particularly at pro-oestrus, when oestrogen concentrations are highest.

(PDF same-day service: $19.90)

Accession: 002139734

Download citation: RISBibTeXText

PMID: 1886090

DOI: 10.1530/jrf.0.0920323



Related references

Regulation of follicular development by diethylstilboestrol in ovaries of immature rats. Journal of Reproduction and Fertility 92(2): 307-321, 1991

Effects of different estrous stages of ovaries and sizes of follicles on generation of bovine embryos in vitro. Theriogenology 33(1): 335, 1990

Removal of intact follicles from immature mice ovaries. Differentiation; Research in Biological Diversity 4(2): 81-84, 1975

Morphometric characterization of immature pig oocytes obtained from antral follicles of different sizes. 1 Congresso Iberico Reproducao Animal, Estoril, Portugal 3 a 6 de Julho de 1997, Volume 2: comunicacoes livres comunicaciones: 225-228, 1997

Histological observation of the development of follicles and follicular atresia in immature rat ovaries. Acta Medica Okayama 61(5): 283-298, 2007

Restoration of fecundity to sterilized mouse ovaries by transferring immature follicles. Journal of Physiology (Cambridge) 420: 11P, 1990

The actions of chorionic gonadotrophin and testosterone on the follicles of various sizes in the ovary of the hypophysectomized immature rat. Acta Physiologica et Pharmacologica Neerlandica 4(1): 82-95, 1955

Comparative proteomic analysis of primordial follicles from ovaries of immature and aged rats. Systems Biology in Reproductive Medicine 61(6): 367-375, 2016

Effects of sex organ stimulating hormones on ovum development within follicles of immature mouse ovaries in cultivation. Korean Journal of Zoology 13(4): 124, 1970

Histological studies on onset of meiosis and on the time of appearance of follicles in different stages in immature rabbit ovaries. Journal of the Faculty of Applied Biological Science Hiroshima University 28(1-2): 15-26, 1989

Comparison of the developmental potential of 2-week-old preantral follicles derived from vitrified ovarian tissue slices, vitrified whole ovaries and vitrified/transplanted newborn mouse ovaries using the metal surface method. Bmc Biotechnology 8: 38-38, 2008

Growth differentiation factor-9 increases the number of primary and small preantral follicles and theca cell marker CYP17 in ovaries of immature rats in vivo. Biology of Reproduction 62(Supplement 1): 279, 2000

In vitro maturation potential of sheep oocytes isolated from follicles of different sizes. Indian Journal of Small Ruminants 5(2): 90-93, 1999

Proliferative potential comparison of keratinocytes from different fragment of hair follicles. Zhonghua Pifuke Zazhi 32(SUPPL ): 4, June, 1999