+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Intra- and extracellular amino acid concentrations in portacaval-shunted rabbits. Role of hyperammonemia and effects of branched-chain amino acid-enriched parenteral nutrition



Intra- and extracellular amino acid concentrations in portacaval-shunted rabbits. Role of hyperammonemia and effects of branched-chain amino acid-enriched parenteral nutrition



Zeitschrift fur Ernahrungswissenschaft 48(2): 149-172



Intra- and extracellular amino acid concentrations were measured in rabbits in order to elucidate the possible role of hyperammonemia in lowering the postabsorptive plasma levels of branched-chain amino acids (BCAA) and to assess the effects of BCAA-enriched total parenteral nutrition (TPN) on the amino acid pattern of muscle. The pathophysiological part of this paper deals with portacaval anastomosis (PCA) and is aimed at substantiating or rejecting our hypothesis that excessive ammonia-by stimulating glutamine synthesis-reduces the intracellular glutamate pool which is then restored, at least in part, by an intensified BCAA degradation. Regarding infusion therapy, we were mainly interested in whether an amino acid solution adapted to the metabolism in liver cirrhosis causes an accumulation of BCAA in muscle or modifies the intracellular content of glutamate and glutamine. Eighteen rabbits did not undergo surgery and served as controls (group A), while 30 were given a portacaval end-to-side anastomosis (group B). Two weeks after creating the PCA, venous blood samples were taken and muscle biopsies (Bergström's technique) were performed postabsorptively. An 18-h TPN was then started, the regimen administered included dextrose, fat and, in addition, either a conventional (group B1, n = 15) or an adapted amino acid solution (group B2, n = 15). We obtained second blood specimens and muscle biopsies at the end of the infusion period. With the control animals, the same time schedule for blood sampling and muscle biopsies was followed. Fourteen days after the operation, the PCA rabbits displayed a mean plasma ammonia level 5.1 times higher than that measured in the controls (p less than or equal to 0.001). Conventional blood chemistry did not reveal any impairment of liver cell integrity or over-all hepatic function, whereas the nutritional state of the shunted animals worsened, as indicated by body weight and biochemical variables. Since in the PCA rabbits, the total amino acid pools of muscle and plasma were seen to be increased and decreased, respectively, the results concerning the individual amino acids are given in terms of both the absolute and percentage values, the latter more often revealing high levels of statistical significance. PCA induced a marked rise in the intra- and extracellular concentrations of glutamine, while the values of glutamate and alanine showed a decline in muscle and plasma. The extracellular levels of methionine, phenylalanine, and tyrosine were raised, while those of the BCAA were diminished.(ABSTRACT TRUNCATED AT 400 WORDS)

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002143197

Download citation: RISBibTeXText

PMID: 2569793

DOI: 10.1007/bf02030130


Related references

Intracellular and extracellular amino acid concentrations in portacaval shunted rabbits role of hyperammonemia and effects of branched chain amino acid enriched parenteral nutrition. Zeitschrift fuer Ernaehrungswissenschaft 28(2): 149-172, 1989

Effects of branched chain amino acid-enriched total parenteral nutrition on amino acid utilization in rats bearing Yoshida sarcoma. Cancer Research 48(10): 2698-2702, 1988

Effects of total parenteral nutrition enriched with branched chain amino acid infusion on liver function and serum amino acid pattern in dogs undergoing hepatectomy. Ricerca in Clinica e in Laboratorio 15(1): 79-88, 1985

Intracellular and extracellular amino acid concentrations in ammonium infused rats evidence that hyperammonemia reduces branched chain amino acid levels. Kleinberger, G , P Ferenci, P Riederer And H Thaler (Ed ) Advances in Hepatic Encephalopathy And Urea Cycle Diseases 5th International Symposium on Ammonia, Semmering, Austria, May 16-19, Xii+769p S Karger: Basel, Switzerland; New York, N Y , Usa Illus 552-554, 1984

The effect of enriched branched chain amino acid solution on amino acid metabolism during perioperative total parenteral nutrition support. Zhonghua Wai Ke Za Zhi 27(12): 756, 1989

Metabolic effects of arginine and branched chain amino acid enriched total parenteral nutrition on walker 256 carcinosarcoma. FASEB Journal 4(4): A1177, 1990

The effect of the administration of an amino acid solution enriched with branched-chain amino acids on the nutritional assessment of patients with stress who require total parenteral nutrition. Nutricion Hospitalaria 8(4): 225-241, 1993

Alanylglutamine-enriched total parenteral nutrition improves protein metabolism more than branched chain amino acid-enriched total parenteral nutrition in protracted peritonitis. Journal of Trauma 42(2): 183-190, 1997

The effect of branched-chain amino acid-enriched parenteral nutrition on gut permeability. Nutrition 12(3): 176-179, 1996

Acute hyperammonemia activates branched-chain amino acid catabolism and decreases their extracellular concentrations: different sensitivity of red and white muscle. Amino Acids 40(2): 575-584, 2011

Branched chain amino acid enriched parenteral nutrition in surgical intensive care patients. Clinical Nutrition 5(3): 167-170, 1986

Evaluation of an infusion solution enriched with branched chain amino acid in parenteral nutrition of rats. Journal of Nutrition 110(4): 710-719, 1980

Evaluation of an Infusion Solution Enriched with Branched Chain Amino Acid in Parenteral Nutrition of Rats. The Journal of Nutrition 110(4): 710-719, 1980

Selection of an optimal branched chain amino acid enriched parenteral nutrition solution for the post traumatic rat. Clinical Research 29(2): 500A, 1981

Mechanism of decreases in blood branched chain amino acid levels in liver cirrhosis 3. effects of administration of short chain fatty acid on blood branched chain amino acid concentrations in rats. Acta Scholae Medicinalis Universitatis in Gifu 29(4): 642-651, 1981