Isolation of Staphylococcus hyicus subsp. hyicus from pigs affected with exudative epidermitis and experimental infection of piglets with isolates
Sato, H.; Tanabe, T.; Nakanowatari, M.; Oyama, J.; Yamazaki, N.; Yoshikawa, H.; Yoshikawa, T.; Koyama, H.; Saito, H.
Kitasato Archives of Experimental Medicine 63(2-3): 119-130
ISSN/ISBN: 0023-1924 PMID: 2096257 Accession: 002146279
Five strains of S. hyicus subsp. hyicus were isolated: 3 strains (P-1, P-2 and P-3) from the crust on the body surface of 6-month-old pigs on a farm in Aomori prefecture, and 2 (P-5 and P-6) from both the crust on the body surface and the joint of a 1-month-old piglet with exudative epidermitis (EE) on another farm. The characterization of the isolates and the experimental infection of the piglets with strain P-1 were carried out. Subcutaneous inoculation with the bacterial suspension (1010 CFU) produced EE in all 9 piglets. Eight of them had exudation and exfoliation within 24 h of infection. Histopathologically, disappearance of stratum corneum and necrosis with vacuolar degeneration of prickle cells were pronounced in the epidermis.