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Isolation of the 66-kilodalton polypeptide from promastigotes of Leishmania donovani as a ligand molecule for binding to macrophages

Isolation of the 66-kilodalton polypeptide from promastigotes of Leishmania donovani as a ligand molecule for binding to macrophages

Japanese Journal of Medical Science & Biology 44(3): 109-121

A 66 000 MW major plasma membrane-associated molecule of promastigotes of L. donovani (UR6) was purified by affinity chromatography. The immunoreactivity of the molecule was lost upon exposure to heat or treatment with trypsin. The metaperiodate oxidation significantly reduced its immunoreactivity. The molecule is, therefore, glycoprotein in nature. With a fluorescent probe, the molecule was found to be located on the tip of the flagellum and on the kinetoplast.

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Accession: 002146583

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PMID: 1784082

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