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Monoclonal antibody-based sandwich ELISA for detection of serotype-specific antigens of Actinobacillus (Haemophilus) pleuropneumoniae



Monoclonal antibody-based sandwich ELISA for detection of serotype-specific antigens of Actinobacillus (Haemophilus) pleuropneumoniae



Proceedings, International Pig Veterinary Society, 11th Congress, July 1-5, 1990, Lausanne, Switzerland: 2



A method is described for the detection of A. pleuropneumoniae serotype-specific antigens applying sandwich ELISA, using rabbit polyclonal antiserum against serotype 2, strain SH-15, and monoclonal antibody, 2 Mab-5, specific for serotype 2 strains, as capture and second antibodies respectively. When the sandwich ELISA technique was applied to cotton swab nasal and lung samples from pigs infected with serotype 2 A. pleuropneumoniae, there was an exact correlation with the culture method.

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Accession: 002164522

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