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Mutational analysis of parasite trypanothione reductase: acquisition of glutathione reductase activity in a triple mutant



Mutational analysis of parasite trypanothione reductase: acquisition of glutathione reductase activity in a triple mutant



Biochemistry, 3011: 2761-2767



African trypanosomes contain a cyclic derivative of oxidized glutathione, N1,N8-bis(glutathionyl)spermidine, termed trypanothione. This is the substrate for the parasite enzyme trypanothione reductase, a key enzyme in disulfide/dithiol redox balance and a target enzyme for trypanocidal therapy. Trypanothione reductase from these and related trypanosomatid parasites is structurally homologous to host glutathione reductase but the 2 enzymes show mutually exclusive substrate specificities.

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Accession: 002166521

Download citation: RISBibTeXText

PMID: 2007114

DOI: 10.1021/bi00225a004


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