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Na+-dependent transport of alanine and serine by liver plasma-membrane vesicles from rats fed a low-protein or a high-protein diet



Na+-dependent transport of alanine and serine by liver plasma-membrane vesicles from rats fed a low-protein or a high-protein diet



Biochimica et Biophysica Acta, Biomembranes 1026(1): 1-12



Plasma-membrane vesicles prepared from the livers of rats fed a low-(LP) or a high-protein (HP) diet exhibited Na+-dependent active transport of alanine and serine. The process gave apparent kinetic parameters compatible with a single saturable component for both amino acids. Na/K-ATPase (EC 3.6.1.37), marker of the basolateral domain of the hepatocyte plasma-membrane, was chosen as reference for the expression of amino acid transport in vesicle preparations.

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Accession: 002167138

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PMID: 2165806

DOI: 10.1016/0005-2736(90)90325-i


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