+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Nucleotide sequences of genome segments S8, encoding a capsid protein, and S10, encoding a 36K protein, of rice gall dwarf virus



Nucleotide sequences of genome segments S8, encoding a capsid protein, and S10, encoding a 36K protein, of rice gall dwarf virus



Journal of General Virology 72: 2837-2842



The nucleotide sequences of DNAs complementary to the eighth (S8) and the tenth (S10) largest of the 12 genome segments of rice gall dwarf virus (RGDV) were determined. The S8 and S10 segments consist of 1578 and 1198 nucleotides, each with a single open reading frame extending for 1278 nucleotides from nucleotide 21, and 960 nucleotides from nucleotide 22, respectively. S8 encodes a polypeptide of 426 amino acids with an Mr of 47419. The amino acid sequences of several peptide fragments of the major outer capsid protein reported as 45K were contained in the predicted polypeptide. This protein, renamed the 47K protein, showed high homology with the outer capsid proteins of rice dwarf virus (RDV) and wound tumour virus (WTV); there was 56, 52 and 48% amino acid sequence identity between RGDV and WTV, RGDV and RDV, and RDV and WTV, respectively. S10 had the coding potential for a polypeptide of 320 amino acids with an Mr of 36,095 (36K protein), which exhibits 32% and 35% amino acid sequence identity with the predicted translation product of RDV S9 and the P9 capsid protein encoded by WTV S11, respectively. The conserved terminal sequences 5' GG...GAU 3' which are present in all genome segments of WTV and RDV so far analysed, and in S9 of RGDV, were also found in RGDV S8 and S10. This conserved sequence together with the segment-specific inverted repeats found in the terminal sequence of RGDV S8 and S10 are thus characteristic structures common to all three phytoreoviruses. The nucleotide sequence of the region surrounding the inverted repeats was more similar between RGDV and WTV than between RGDV and RDV.

Please choose payment method:






(PDF emailed within 1 workday: $29.90)

Accession: 002172445

Download citation: RISBibTeXText

PMID: 1940872


Related references

The cDNA cloning and nucleotide sequence of the gene encoding nonstructure protein of rice dwarf virus genome segment 10. Acta Botanica Sinica 35(2): 115-120, 1993

Nucleotide sequences of segments 1, 3 and 4 of the genome of Bombyx mori cypovirus 1 encoding putative capsid proteins VP1, VP3 and VP4, respectively. Journal of General Virology 83(Pt 6): 1477-1482, 2002

Genome segments encoding capsid protein-like variants of Pyrus pyrifolia cryptic virus. Virus Research 240: 64-68, 2017

The minor outer capsid protein P2 of rice gall dwarf virus has a primary structure conserved with, yet is chemically dissimilar to, rice dwarf virus P2, a protein associated with virus infectivity. Archives of Virology 142(10): 2011-2019, 1997

The complete nucleotide sequences of L3 and S7 segments of Ibaraki virus encoding for the major inner capsid proteins, VP3 and VP7. Journal of Veterinary Medical Science 63(1): 73-78, 2001

In vitro but not in planta encapsidation of Rice gall dwarf virus core particles by the outer capsid P8 protein of Rice dwarf virus expressed in transgenic rice plants. Journal of General Plant Pathology 72(3): 186-189, 2006

The sequence and phylogenetic analysis of avian reovirus genome segments M1, M2, and M3 encoding the minor core protein muA, the major outer capsid protein muB, and the nonstructural protein muNS. Journal of Virological Methods 133(2): 146-157, 2006

Complete nucleotide and deduced amino acid sequence of genome segment 5 encoding the outer capsid protein, VP5, of a U.S. isolate of bluetongue virus serotype 11. Virus Research 21(2): 155-162, 1991

The sequences of reovirus serotype 3 genome segments M1 and M3 encoding the minor protein mu 2 and the major nonstructural protein mu NS, respectively. Virology 169(2): 293-304, 1989

Cloning and sequence analysis of a complementary dna encoding the capsid protein of the mav isolate of barley yellow dwarf virus. Nucleic Acids Research 18(15): 4625, 1990

Cloning and sequence analysis of a cDNA encoding the capsid protein of the MAV isolate of barley yellow dwarf virus. Nucleic Acids Research 18(15): 4625-4625, 1990

Cloning and sequence analysis of N gene encoding Akabane virus nucleotide capsid protein. Virologica Sinica 19(1): 18-21, 2004

The outer capsid protein of rice dwarf virus is encoded by genome segment S8. Journal of General Virology 70: 2759-2764, 1989

Nucleotide sequence of cDNA clones encoding the outer capsid protein, VP5, of bluetongue virus serotype 10. Journal of General Virology 67: 957-962, 1986

Nucleotide sequence of the outer capsid protein gene of severe strain of rice dwarf virus. Annals of the Phytopathological Society of Japan 59(4): 438-440, 1993