+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

Prolactin messenger ribonucleic acid levels, prolactin synthesis, and radioimmunoassayable prolactin during the estrous cycle in the golden Syrian hamster



Prolactin messenger ribonucleic acid levels, prolactin synthesis, and radioimmunoassayable prolactin during the estrous cycle in the golden Syrian hamster



Life Sciences 46(13): 909-916



The purpose of this study was to observe the molecular dynamics of pituitary prolactin (PRL) gene expression during the estrous cycle of the Golden Syrian hamster. PRL messenger ribonucleic acid (mRNA) levels, PRL synthesis (3H-PRL in the incubation media or incubated pituitary after a 3 hr incubation with 3H-leucine), and radioimmunoassayable (RIA) PRL (in the incubation media or incubated pituitary after the 3 hr incubation) were measured in the morning (0930-1100 hr) on each day of the cycle. We observed that all of these PRL indices declined or did not change from Day 2 to Day 3 of the cycle. From Day 3 to Day 4 (proestrus), however, PRL mRNA levels increased 33-38% and media 3H-PRL increased 32-42%, while there were no significant changes in pituitary 3H-PRL, or RIA-PRL in the media or pituitary. From Day 4 to Day 1 (estrus) there was a reciprocal change in the levels of 3H-PRL in the pituitary vs. the media, with the former increasing 37-50% and the latter decreasing 25-32%. Pituitary RIA-PRL also increased 45-64% from Day 4 to Day 1 while media RIA-PRL did not change. These data are consistent with the following hypothesis: On the morning of proestrus (Day 4) in the hamster, PRL mRNA levels are elevated compared to those on Day 3, signaling an increase in PRL synthesis. This newly synthesized PRL is shunted into a "readily releasable" pool on the morning of Day 4 (contributing to the afternoon surge of serum PRL), and into a "preferentially stored" pool by the morning of Day 1 (for release in response to cervical stimulation and use as a luteotrophin to maintain early pregnancy should fertilization occur).

Please choose payment method:






(PDF emailed within 0-6 h: $19.90)

Accession: 002196963

Download citation: RISBibTeXText

PMID: 2329917

DOI: 10.1016/0024-3205(90)90092-6


Related references

Prolactin messenger ribonucleic acid concentrations throughout the ovine estrous cycle: assessment relative to prolactin serum and pituitary amounts. Life Sciences 48(12): 1179-1184, 1991

Quantification of prolactin messenger ribonucleic acid, pituitary content and plasma levels of prolactin, and detection of immunoreactive isoforms of prolactin in pituitaries from turkey embryos during ontogeny. Biology of Reproduction 61(3): 757-763, 1999

Thyroid hormone specifically inhibits prolactin synthesis and decreases prolactin messenger ribonucleic acid levels in cultured pituitary cells. Endocrinology 110(5): 1507-1514, 1982

Estradiol increases prolactin synthesis and prolactin messenger ribonucleic acid in selected brain regions in the hypophysectomized female rat. Endocrinology 131(5): 2154-2160, 1992

Prolactin receptor messenger ribonucleic acid expression in the ovary during the rat estrous cycle. Endocrinology 133(6): 2594-2603, 1993

Hypothalamic prolactin receptor messenger ribonucleic acid levels, prolactin signaling, and hyperprolactinemic inhibition of pulsatile luteinizing hormone secretion are dependent on estradiol. Endocrinology 149(4): 1562-1570, 2008

Expression and localization of prolactin receptor messenger ribonucleic acid in red deer ovary during the estrous cycle and pregnancy. Biology of Reproduction 57(4): 865-872, 1997

Possible role of prolactin on bovine luteal function and the gene expression of prolactin and prolactin receptor in corpus luteum throughout the estrous cycle. Biology of Reproduction 68(Suppl. 1): 140, 2003

Human thymocytes express a prolactin-like messenger ribonucleic acid and synthesize bioactive prolactin-like proteins. Endocrinology 131(6): 3019-3026, 1992

Detection of prolactin messenger ribonucleic acid and prolactin-like substance in thyroid glands from patients with Graves disease. Endocrine Journal 47(Suppl August): 196, 2000

Depressed pituitary prolactin mRNA, prolactin synthesis, and prolactin storage after light-deprivation in female hamsters is not due to loss of estrous cyclicity alone. Biology of Reproduction 41(5): 827-834, 1989

Studies on the immunologic potency of standard prolactin in different ria kits for prolactin and serum prolactin levels during the menstrual cycle. Mizuno, M , H Mori And Y Taketani (Ed ) Role Of Prolactin in Human Reproduction; 1st Symposium, Tokyo, Japan, October 4, 1985; 2nd Symposium, Tokyo, Japan, October 3, 1986; 3rd Symposium, October 2, 1987 X+313p S Karger Ag: Basel, Switzerland; New York, New York, Usa Illus 54-63, 1988

Serum LH, FSH and prolactin levels during the estrous cycle in the hamster. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine 145(2): 571-573, 1974

Relationship between estradiol ergocryptine and thyroid hormone effects on prolactin synthesis and prolactin messenger rna levels. Endocrinology 110(5): 1515-1520, 1982

Cyclic amp derivatives increase prolactin synthesis and prolactin messenger rna levels in ergocryptine treated pituitary cells. Endocrinology 110(6): 1957-1963, 1982