+ Site Statistics
+ Resolve Accession
+ Subscribe to Site Feeds
EurekaMag Most Shared ContentMost Shared
EurekaMag PDF Full Text ContentPDF Full Text
+ PDF Full Text
Request PDF Full TextRequest PDF Full Text
Submit PDF Full TextSubmit PDF Full Text
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter
Follow on Google+Follow on Google+
Follow on LinkedInFollow on LinkedIn

+ Translate

Quantitative and qualitative properties of host polymorphonuclear cells during experimentally induced Staphylococcus aureus mastitis in cows

, : Quantitative and qualitative properties of host polymorphonuclear cells during experimentally induced Staphylococcus aureus mastitis in cows. American Journal of Veterinary Research 52(3): 474-479

Polymorphonuclear cells have a critical role in the pathogenesis of bovine mastitis. We have documented that experimentally induced Staphylococcus aureus mastitis is associated with cyclic increase and decrease in the quantity of viable bacteria shed in the milk. Concomitant with this cycling of bacteria is an inverse cycling of the hosts cells within the milk. Such somatic cells were determined to be greater than or equal to 95% polymorphonuclear cells. The quality of these cells was evaluated by measuring their relative efficiency of bacterial killing and phagocytosis at various times during an infection. Host polymorphonuclear cells had as much as 10,000-fold variation in the bactericidal failure rate for staphylococci during cell cycling. The most efficient bactericidal effect was observed at or near the peak of the somatic cell count (SCC). The ability of these cycling cells to ingest fluorescent beads was also quantitated by use of flow cytometry. The percentage of phagocytic polymorphonuclear cells that ingested fluorescent latex beads ranged from 15 to 80% of the total cell population during cell cycling, and tended to be optimal at or near peak SCC. In addition, the average number of beads ingested varied between 1 and 2 particles/polymorphonuclear cell, with as many as 17% of the phagocytic cells ingesting 4 or more beads at maximal efficiency. Polymorphonuclear cells from quarters infected with S aureus varied quantitatively (total SCC) and qualitatively (bactericidal activity and phagocytic ability) during the course of an infection. Not only is the quantity of host's phagocytic cells in the mammary gland central to the defense mechanism against infection, but the biological activation state appears to be equally important. The role of these cells in the pathogenesis of a cycling infection is presented in a model to explain the cyclic nature of mastitis.

(PDF 0-2 workdays service)

Order PDF Full Text


Click here to order any other PDF Full Text

Accession: 002200624

PMID: 2035925

PDF Full Text: Submit

Submit PDF Full Text

No spam - Every submission is manually reviewed

Due to poor quality, we do not accept files from Researchgate

Submitted PDF Full Texts will always be free for everyone
(We only charge for PDFs that we need to acquire)

Select a PDF file:

Other references

San Jose, C.; Asp, N. G.; Burvall, A.; Dahlqvist, A., 1977: Water sorption in lactose hydrolyzed dry milk. Water sorption isotherms for dried lactose-hydrolysed milk were determined at 25 deg C. Compared with normal dried milk, lactose-hydrolysed milk adsorbed less water at water activities <0.2 and up to twice as much water at water activities >...

Peshin, Pk; Krishnamurthy, D; Nigam, Jm; Rama-Kumar, V., 1977: Inguinal hernia in a cow. Haryana veterinarian 16(1): 42-43

Oka, H.; Ikai, Y.; Kawamura, N.; Hayakawa, J.; Yamada, M.; Harada, K. I.; Murata, H.; Suzuki, M.; Nakazawa, H.; Etal, 1991: Purification of food color red no. 106 acid red using high speed countercurrent chromatography. High-speed counter-current chromatography (HSCCC) has been successfully applied to the separation of the components of Food Color Red No. 106 (R-106). The separation was performed using 25 mg of the sample with a two-phase solvent system composed...

Schwartz, J.; Leber, M.D.; Gillis, S.; Giunta, A.; Eldor, A.; Bussel, J.B., 2003: Long term follow-up after splenectomy performed for immune thrombocytopenic purpura (ITP). Splenectomy is the only treatment of ITP known to have "curative" effects in a substantial fraction of patients. However, the true long-term outcome is uncertain and controversial because published series have not adjusted for the durati...

Goffart, H., 1949: Ruben-, Hafer- und Kartoffelnematoden. In this leaflet Goffart deals separately with beet, oat and potato root netnatodes. For each species he gives the life-history, host plants, effects on the principal host, means of spread and control measures. No effective chemical means of contro...

McCarty, M.F., 2007: Gamma-tocopherol may promote effective no synthase function by protecting tetrahydrobiopterin from peroxynitrite. Oxidation of tetrahydrobiopterin by peroxynitrite in oxidant-stressed endothelium compromises nitric oxide synthase (eNOS) activity while amplifying superoxide production; this mechanism contributes prominently to the endothelial dysfunction that...

Krietsch, P., 1993: History of the Prosectors Office at the Charite Hospital, Berlin 5 Johann Heinrich Meckel von Hemsbach, Prosector of Charite Hospital 1852-1856. Zentralblatt Fuer Pathologie. 139(4-5): 391-399

Vanhatalo, S.; Soinila, S., 2001: Evidence for nodose ganglion as the source of innervation to the anterior lobe of the pituitary gland. Recent studies have provided convincing evidence for the presence of peptidergic nerve fibers in the pituitary anterior lobe in several animal species. This study was aimed at elucidating the origin of this innervation by neuro-anatomical tracing,...

Russell, Ewb, 1983: Pollen analysis of past vegetation at Point Reyes National Seashore, California. Madrono 30(1): 1-11

Khudoley K. M.; Rzhonsnitskaya M. A.; Nikiforova O. I., 1979: Palaeobiogeography of the Pacific Ocean in the Phanerozoic. Abstracts of Papers - Pacific Science Congress 14(1): 28-29