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Quantitative assessment of gamma -linolenic acid in human blood and plasma with capillary gas chromatography


, : Quantitative assessment of gamma -linolenic acid in human blood and plasma with capillary gas chromatography. Journal of Chromatography, Biomedical Applications 528(2): 447-452

A sensitive technique for the assessment of gamma -linolenic acid at the nanomolar level in 0.4 ml of blood or blood plasma is described. Circulating lipid pools were extracted with chloroform-methanol. Purification and isolation of the various lipid classes was done using one-dimensional thin-layer chromatography. Quantitation of the content of gamma -linolenic acid in the various lipid classes was performed with capillary gas chromatography (GC).

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Accession: 002200641

PMID: 2166745

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