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Quantitative determination of leiocarposide in Solidago virgaurea L

, : Quantitative determination of leiocarposide in Solidago virgaurea L. Pharmazie 45(5): 380-381

A new method to determine leiocarposide content in S. virgaurea based on the Emerson reaction is described. Leiocarposide, when reacted with aminopyrazolone and an oxidizer in alkaline conditions, gives a product stable in chloroform with a UV absorption maximum at 455 nm. There was a close linear relationship between leiocarposide concentration (C, in the range 0.002-0.020 mg/ml) and the extinction coefficient (E), viz. C = 0.0414573 E.

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Schmidt, R.J.; Emara, M.G.; Kung, L., 2008: The use of a quantitative real-time polymerase chain reaction assay for identification and enumeration of Lactobacillus buchneri in silage. Aims: To detect and quantify <i>Lactobacillus buchneri</i> in plant samples with the aid of polymerase chain reaction (PCR) methods. Methods and Results: DNA from silage samples spiked with different amounts of <i>L. buchneri<...

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