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Quantitative determination of the gibberellins GA3 and GA1 in spruce (Picea abies ) needles by a highly specific enzyme immunoassay


, : Quantitative determination of the gibberellins GA3 and GA1 in spruce (Picea abies ) needles by a highly specific enzyme immunoassay. Journal of Plant Physiology 139(2): 229-234

The gibberellins GA1 and GA3 were quantitatively determined in needle extracts from spruce (Picea abies [L.]). The phytohormones were purified by several extraction procedures and by HPLC, and detected by a highly sensitive enzyme-linked immunosorbent assay (ELISA). The performance of the EIA was validated by monitoring the parallelism of the logit-log transformed curves of standard and extract dilutions, and by spiking the samples with known amounts of GA. The concentrations of GA, were higher than GA1 in all samples tested so far. Furthermore, GA3 levels in needles of strongly damaged trees with yellowing symptoms exceeded those of healthy trees.

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Accession: 002200711

DOI: 10.1016/S0176-1617(11)80613-9

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