+ Site Statistics
+ Search Articles
+ PDF Full Text Service
How our service works
Request PDF Full Text
+ Follow Us
Follow on Facebook
Follow on Twitter
Follow on LinkedIn
+ Subscribe to Site Feeds
Most Shared
PDF Full Text
+ Translate
+ Recently Requested

RNA editing in trans-splicing intron sequences of nad2 mRNAs in Oenothera mitochondria

RNA editing in trans-splicing intron sequences of nad2 mRNAs in Oenothera mitochondria

Journal of Biological Chemistry 267(11): 7615-7623

The complete open reading frame of subunit 2 of the NADH dehydrogenase in Oenothera mitochondria is split into five exons. The first two and the last three exons are encoded in distant genomic locations and are transcribed separately. Three tRNA genes coding for tRNA(Cys), tRNA(Asn), and tRNA(Tyr) are located upstream of the terminal three exons c, d, and e. The genomic distance, the interspersed tRNA genes, and the group II intron sequences flanking the two separated exons suggest trans-splicing to be required to connect exons b and c. Maturation of the mRNA includes RNA editing at 36 sites in the open reading frame. Three RNA editing events are observed in the split group II intron sequences. Two of these events allow after editing additional base pairings in the secondary structure, one in the stem of domain I, the other in the putative trans-pairing region of domain IV. These RNA editings may thus be involved in the trans-splicing reaction.

Please choose payment method:

(PDF emailed within 1 workday: $29.90)

Accession: 002201143

Download citation: RISBibTeXText

PMID: 1559998

Related references

Trans splicing in Oenothera mitochondria: nad1 mRNAs are edited in exon and trans-splicing group II intron sequences. Cell Cambridge 65(3): 473-482, 1991

Trans splicing in oenothera mitochondria nad1 messenger rnas are edited in exon and trans splicing group ii intron sequences. Cell 65(3): 473-482, 1991

Cis- and trans-splicing and RNA editing are required for the expression of nad2 in wheat mitochondria. Molecular and General Genetics 258(5): 503-511, 1998

Editing of pre-mRNAs can occur before cis- and trans-splicing in Petunia mitochondria. Molecular and Cellular Biology 11(8): 4274-4277, 1991

The PPR protein SLOW GROWTH 4 is involved in editing of nad4 and affects the splicing of nad2 intron 1. Plant Molecular Biology (): -, 2016

Group I-intron trans-splicing and mRNA editing in the mitochondria of placozoan animals. Trends in Genetics 25(9): 381-386, 2009

RNA editing of a group II intron in Oenothera as a prerequisite for splicing. Molecular and general genetics: MGG 246(6): 739-744, 1995

RNA editing makes mistakes in plant mitochondria: editing loses sense in transcripts of a rps19 pseudogene and in creating stop codons in coxI and rps3 mRNAs of Oenothera. Nucleic Acids Research 19(24): 6923-6928, 1991

Loss of RNA editing of rps1 sequences in Oenothera mitochondria. Current genetics0(5): 455-460, 1996

Intron RNA editing is essential for splicing in plant mitochondria. Nucleic Acids Research 38(20): 7112-7121, 2011

Editing of precursor messenger rnas can occur before cis splicing and trans splicing in petunia mitochondria. Molecular & Cellular Biology 11(8): 4274-4277, 1991

RNA editing in ATPase subunit 6 mRNAs in Oenothera mitochondria. A new termination codon shortens the reading frame by 35 amino acids. Febs Letters 295(1-3): 97-101, 1991

Relationship between RNA splicing and exon editing near intron junctions in wheat mitochondria. Physiologia plantarum 129(1): 23-33, 2007

Trans-splicing and RNA editing of LSU rRNA in Diplonema mitochondria. Nucleic Acids Research 42(4): 2660-2672, 2014

Alternative splicing of fibronectin mRNAs in chondrosarcoma cells: role of far upstream intron sequences. Journal of Cellular Biochemistry 90(4): 709-718, 2003